- P-ISSN 1225-0163
- E-ISSN 2288-8985
- Apply for Authority
- P-ISSN1225-0163
- E-ISSN2288-8985
- SCOPUS, ESCI, KCI
Article Detail
Home > Article Detail
Development and validation of an analytical method for the determination of lepimectin residues by HPLC-PDA
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
2013, v.26 no.2, pp.142-153
https://doi.org/10.5806/AST.2013.26.2.142
&
(2013). Development and validation of an analytical method for the determination of lepimectin residues by HPLC-PDA. Analytical Science and Technology, 26(2), 142-153, https://doi.org/10.5806/AST.2013.26.2.142
- Downloaded
- Viewed
Abstract
A new, rapid, and simple analytical method was developed and validated using high performance liquid chromatograph-photodiode array detector (HPLC-PAD) for the determination of lepimectin residues in agricultural commodities. The lepimectin residues in samples were extracted with methanol, partitioned with dichloromethane, and then purified with glass column filled with subsequently to aminopropyl (NH2) solid phase extraction (SPE) cartridge. The purified samples were detected using HPLC-PAD. Correlation coefficient (R2)of both lepimectin A3 and A4 solutions were 0.9999. The method was validated using cucumber spiked with lepimectin at 0.02 and 0.2 mg/kg and pepper, mandarin, hulled rice, potato, soybean at 0.02 and 0.5 mg/kg. Average recoveries were 76.0~114.8% with relative standard deviation less than 10%, and limit of detection (LOD) and limit of quantification (LOQ) were 0.005 and 0.01 mg/kg, respectively. The result of recoveries and overall coefficient of variation of a laboratory results in Gwangju regional KFDA and Daejeon regional KFDA was followed with Codex guideline (CAC/GL 40). Therefore, developed method in this study is accurate,rapid, and appropriate for lepimectin determination and will be used to keep safety of lepimectin residues in agricultural products.
- keywords
- lepimectin, HPLC-PDA, agricultural commodities, LOD, LOQ
Reference
1
Alanwood, http://alanwood.net/pesticides/index
2
KFDA. Korea Food Code. Korea Food and DrugAdministration, Seoul, Korea (2011).
3
2012 작물보호제(농약) 지침서. 한국작물보호협회 (2012).
4
M. H. Jeong, S. S. Hong, K. H. Park, J. E. Park, S. J. Kwack, Y. B. Kim, B. S. Han and W. C. Son, Korean Journal of Pesticide Science, 15(2), 218-229 (2011).
5
KFDA, http://fse.foodnara.go.kr/residue/pesticides/ pesticides_info_view.jsp#ADI
6
AOAC, Pesticide and industrial chemical residues, In official method of analysis, 17th ed, 1-88, AOAC International Arlington, VA, USA (2000).
7
US FDA, Pesticide Analytical Manual, Vol 1: Multi residue Methods(3rd ed.) US Food and Drug Administration, USA (1999).
8
식품공전 잔류농약 분석법 실무해설서, 이영득, KFDA (2012).
9
Codex Alimentarius Commission, Guidelines on good laboratory practice in residue analysis, CAC/GL 40- 1993, Rome. Italy (2003).
10
S. J. Lee, Y. H. Kim, L. W. Song, Y. S. Hwang, J. D. Lim, E. H. Sohn, M. H. Im, J. A. Do, J. H. Oh, K. S. Kwon, J. K. Lee, Y. D. Lee and M. G. Choung, Korean Journal of Pesticide Science, 15(3), 254-268 (2011).
11
C. H. Kwon, M. H. Chang, M. H. Im, D. I. Choi, S. C. Jung, J. Y. Lee, Y. D. Yu, J. O. Lee and M. K. Hong, Anal. Sci. Technol., 21(6), 518-525 (2008).
Other articles from this issue
- Contents characteristics of polybrominated diphenyl ethers (PBDEs) in indoor household dust
- The quantitative analysis of combustive gases on fire by remote passive open path FT-IR spectrometer
- Error factors and uncertainty measurement for determinations of amino acid in beef bone extract
- Determination of selenium in grains by hydride generation-ICP/MS
- Development and validation of an analytical method for the determination of lepimectin residues by HPLC-PDA
- Development and validation of an analytical method for pyrimisulfan determination in agricultural commodities by LC-MS/MS
- more
Recommanded Articles
This website recommended the use the Chrome browser for journal website.