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Screening method for amines by derivatization reaction on TLC
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
2013, v.26 no.4, pp.228-234
https://doi.org/10.5806/AST.2013.26.4.228
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(2013). Screening method for amines by derivatization reaction on TLC. Analytical Science and Technology, 26(4), 228-234, https://doi.org/10.5806/AST.2013.26.4.228
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Abstract
Methamphetamine is an amine-containing illegal drug and is distributed unlawfully in South Korea. Finding a rapid, convenient and semi-quantitative determination method for methamphetamine is a very importantissue in the area of forensic drug testing. As an effort to develop new screening method, the reactions betweenthree organic compounds which are structurally similar to methamphetamine and N-(9-fluorenylmethoxycarbonyloxy)succinimide (FMOC-NHS) were performed on silica gel (SiO2) TLC plates. Three reference compounds weresynthesized and used for the identification, comparison and study of the limit of detection (LOD) of the productsobtained from a direct reaction on a TLC plate. As a result, FMOC-NHS as a derivatization reagent generatedcompounds containing highly UV-active functional groups on the TLC plate after reacting with primary- andsecondary amines. In the experiment 2D the LOD of amines was in the range of 0.045 and 0.01 mg/mL (2 μL/spot), and in 1D the LOD was in the range of 0.002 and 0.007 mg/mL (2 μL/spot). The LODs of the compoundstested were dependent on the concentration of the derivatizing reagent.
- keywords
- TLC, derivatization with FMOC-NHS, LOD, amphetamine, UV
Reference
1
강규복, ‘마약류범죄에 대한 경찰수사 효율화 방안에 관한 연구’ 석사학위논문, 원광대 행정대학원, 2008.
2
조성권, 마약연구, 3, 1-12 (2006).
3
B. Levine, In ‘Principles of Forensic Toxicology’, 3rd Ed., AACCPress, Washington DC (2010).
4
E. Hahn-Deinstrop, In ‘Applied Thin-layer chromatography’, 2nd Ed., WILEY, Germany (2007).
5
E. Stahl, In ‘Thin-layer chromatography’, 2nd Ed., Springer-Verlag, Berlin (1969).
6
R. Liu and D. Gadzala, In ‘Handbook of drug analysis: applications in forensic and clinical laboratories’, p367, Washington, DC, American Chemical Society (1997).
7
R. Ad. Zeeuw, J. Hartstra and J. P. Franke, J. Chromatogr. A, 674, 3-13 (1994).
8
R. L. Brunelle and M. J. Pro, JAOAC, 55, 823 (1972).
9
K. E. Toole, S. Fu, G. S. Ronald, N. Kraymen and S. Taflaga, Microgram J., 9(1), 27-32 (2012).
10
S. Udenfriend, S. Stein, P. Bohlen, W. Dairman, W. Leimgruber and M. Weigele. Science, 178, 871-872 (1972).
11
N. Kato and A. Ogamo, Sci. Justice, 41(4), 239-244 (2001).
12
G. Gubitz and R. Wintersteiger, J. Anal. Toxicol., 4(3), 141-143 (1980).
13
S. B. Gock and V. A. Skrinska, Clin. Lab. Sci., 18, 114- 118 (2005).
14
S. B. Gock and V. A. Skrinska, Clin. Lab. Sci., 18, 119- 123 (2005).
15
S. Wawrzycki, E. Pyra and B. Wawrzycki, J. Planar Chromatogr., 14, 21-23 (2001).
16
P. J. Ryan, R. Lesicki and J. Roberts, J. Chromatogr., 465, 448-450 (1989).
17
R. M. Linares, J. H. Ayala, A. M. Afonso and V. Gonzalez, Analyst, 123, 725-729 (1998).
18
C. R. Clark and M. M. Wells, J. Chromatogr. Sci., 16, 332-339 (1978).
19
B. M. Farrell and T. M. Jefferies, J. Chromatogr., 272, 111-128 (1983).
20
P. Leroy, A. Nicolas and A. Moreau, J. Chromatogr., 282, 561-569 (1983).
21
G. Maeder, M. Pelletier and W. Haerdi, J. Chromatogr., 593, 9-14 (1992).
22
D. Kazmierczak, W. Ciesielski and R. Zakrzewski, J. Liq. Chromatogr. R. T., 29, 2425-2436 (2006).
23
G. Maeder, M. Pelletier and W. Haerdi, J. Chromatogr., 593, 9-14 (1992).
24
R. Wintersteiger, G. Giibitz and A. Hartinger, Chromatographia, 13(5), 291-294 (1980).
25
J. M. Stoddard, L. Nguyen, H. Mata-Chavez and K. Nguyen, Chem. Commun., 1240-1241 (2007).
26
L. Williams, Chem. Commun., 435-436 (2000).
27
Z. Harduf, T. Nir and B. J. Juven, J. Chromatogr., 437(2), 379-86 (1988).
28
N. Jinkui and D. S. Lawrence, J. Biol. Chem., 272(3), 1493-1499 (1997).
29
H. Kazuhito, K. Mizuho, K. Michiko, F. Miho, T. Shohei and K. Munetaka Synthesis, 12, 1931-1933 (2006).
30
M. Shimizu and M. Sodeoka, Org. Lett., 9(25), 5231- 5234 (2007).
31
H. M. L. Davies and V. Chandrasekar, Angew. Chem. Int. Ed., 41(12), 2197-2199 (2002).
32
S.-W. Choi, S.-G. Lee and H.-I. Lee, Kor. J. Sci. Crim. Investig., 6, 265-270 (2012).
33
N. Kato, S. Fujita, H. Ohta, M. Fukuba, A. Toriba and K. Hayakawa, J. Forensic. Sci., 53(6), 1367-1371 (2008).
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