- P-ISSN 1225-0163
- E-ISSN 2288-8985
- P-ISSN1225-0163
- E-ISSN2288-8985
- SCOPUS, ESCI, KCI
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Validation of LC-MS/MS method for determination of ginsenoside Rg1 in human plasma
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
2013, v.26 no.4, pp.221-227
https://doi.org/10.5806/AST.2013.26.4.221
&
(2013). Validation of LC-MS/MS method for determination of ginsenoside Rg1 in human plasma. Analytical Science and Technology, 26(4), 221-227, https://doi.org/10.5806/AST.2013.26.4.221
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Abstract
A sensitive and selective liquid chromatography-tandem mass spectrometry (LC-MS/MS) was developed for the investigation of the ginsenoside Rg1 in human plasma. After addition of internal standard (digoxin), plasma was diluted with acetone and methanol (80:20), the supernatant was concentrated and analyzed by LC-MS/MS. The optimal chromatographic separation was achieved on an Agilent Eclipse XDB-C18 column (4.6 × 150 mm, 5 μm) with a mobile phase of 0.1% formic acid in water and 0.1% formic acid in methanol at a flow rate of 0.9 mL/min gradient mode. The standard calibration curve for ginsenoside Rg1 was linear (r2=0.9995) over the concentration range 1~500 ng/mL in human plasma. The intra- and inter-day precision over the concentration range of ginsenoside Rg1 was lower than 7.53% (correlation of variance, CV), and accuracy exceeded 98.28%. This LC-MS/MS assay of ginsenoside Rg1 in human plasma is applicable for quantifying in the pharmacokinetic study.
- keywords
- ginsenoside Rg1, LC-MS/MS, validation, human plasma
Reference
1
J. T. Baker, R. P. Borris, B. Carté, G. A. Cordell, D. D. Soejarto, G. M. Cragg, M. P. Gupta, M. M. Iwu, D. R. Madulid and V. E. Tyler, J. Nat. Prod., 58(9), 1325- 1357 (1995).
2
C. S. Yuan, C. Z. Wang, S. M. Wicks and L. W. Qi, J. Ginseng Res., 34(3), 160-167 (2010).
3
M. Mochizuki, Y. C. Yoo, K. Matsuzawa, K. Sato, I. Saiki, S. Tono-oka, K. Samukawa and I. Azuma, Biol. Pharm. Bull., 18(9), 1197-1202 (1995).
4
T. Yokozawa, T. Kobayashi, H. Oura, and Y. Kawashima, Chem. Pharm. Bull., 33(2), 869-872 (1985).
5
K. Takagi, H. Saito and H. Nabata, Jpn. J. Pharmacol., 22(2), 245-249 (1972).
6
P. M. Stavro, M. Woo, T. F. Heim, L. A. Leiter and V. Vuksan, Hypertension, 46(2), 406-411 (2005).
7
C. Pan, Y. Huo, X. An, G. Singh, M. Chen, Z. Yang, J. Pu and J. Li, Vascul. Pharmacol., 56(3-4), 150-158 (2012).
8
H. Saito, Y. Yoshida,and K. Takagi, Jpn. J. Pharmacol., 24(1), 119-127 (1974).
9
B. X. Wang, J. C. Cui, A. J. Liu and S. K. Wu, J. Tradit. Chin. Med., 3(2), 89-94 (1983).
10
H. Matsuda, K. Samukawa and M. Kubo, Planta Med., 56(1), 19-23 (1990).
11
P. Hu, G. A. Luo, Q. Wang, Z. Z. Zhao, W. Wang and Z. H. Jiang, Arch. Pharm. Res., 31(10), 1265-1273 (2008).
12
‘건강기능식품의 기준 및 규격 전면 개정(식품의약품 안정청고시 제 2008-12호)’, 식품의약품안전청.
13
‘인삼 및 인삼제품-진세노사이드(Rb1, Rg1)함량 측정 -고속액체크로마토그래프법(KS H 2153)’, 산업자원 부 기술표준원.
14
H. Oura, S. Hiai, Y. Odaka and T. Yokozawa, J. Biochem., 77(5), 1057-1065 (1975).
15
O. Sticher and F. Soldati, Planta Med., 36(1), 30-42 (1979).
16
T. T. Yip, C. N. Lau, P. P. But and Y. C. Kong, Am. J. Chin. Med., 13(1-4), 77-88 (1985).
17
N. Fuzzati, J. Chromatogr. B., 812(1-2), 119-133 (2004).
18
S. I. Jeong, C. S. Kim, Y. G. Lee, H. S. Lee and I. K. Kim, Anal. Sci. Technol., 11(5), 404-407 (1998).
19
S. I. Jeong, C. S. Kim, N. W. Lee, K. J. Choi, Y. G. Lee and I. K. Kim, Anal. Sci. Technol. 11(6), 436-439 (1998).
20
W. Chen, Y. Dang and C. Zhu, Chin. Med., 5, 12 (2010).
21
J. Sun, G. Wang, X. Haitang, L. Hao, P. Guoyu and I. Tucker, J. pharmaceut. Biomed., 38(1), 126-132 (2005).
22
M. Song, S. Zhang, X. Xu, T. Hang and L. Jia, J. Chromatogr. B., 878(32), 3331-3337 (2010).
23
L. Liu, J.Huang, X. Hu, K. Li and C. Sun J. Chromatogr. B., 879(22), 2011-2017 (2011).
24
J. Zhao, C. Su, C. Yang, M. Liu, L. Tang, W. Su, Z. and Liu J. Pharm. Biomed. Anal., 64-65, 94-97 (2012).
25
D. N. Patel, H. S. Lin and H. L. Koh J. Mass Spectrum., 47, 1510-1517 (2012).
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