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- E-ISSN 2288-8985
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Validation of LC-MS/MS method for determination of ginsenoside Rg1 in human plasma
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
2013, v.26 no.4, pp.221-227
https://doi.org/10.5806/AST.2013.26.4.221
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(2013). Validation of LC-MS/MS method for determination of ginsenoside Rg1 in human plasma. Analytical Science and Technology, 26(4), 221-227, https://doi.org/10.5806/AST.2013.26.4.221
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Abstract
A sensitive and selective liquid chromatography-tandem mass spectrometry (LC-MS/MS) was developed for the investigation of the ginsenoside Rg1 in human plasma. After addition of internal standard (digoxin), plasma was diluted with acetone and methanol (80:20), the supernatant was concentrated and analyzed by LC-MS/MS. The optimal chromatographic separation was achieved on an Agilent Eclipse XDB-C18 column (4.6 × 150 mm, 5 μm) with a mobile phase of 0.1% formic acid in water and 0.1% formic acid in methanol at a flow rate of 0.9 mL/min gradient mode. The standard calibration curve for ginsenoside Rg1 was linear (r2=0.9995) over the concentration range 1~500 ng/mL in human plasma. The intra- and inter-day precision over the concentration range of ginsenoside Rg1 was lower than 7.53% (correlation of variance, CV), and accuracy exceeded 98.28%. This LC-MS/MS assay of ginsenoside Rg1 in human plasma is applicable for quantifying in the pharmacokinetic study.
- keywords
- ginsenoside Rg1, LC-MS/MS, validation, human plasma
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