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- E-ISSN 2288-8985
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Development of HPLC method for differentiation of three parts of mulberry tree
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
2017, v.30 no.3, pp.130-137
https://doi.org/10.5806/AST.2017.30.3.130
(Annexed Food Technology Research Institute)
(Annexed Food Technology Research Institute)
(Jilin Agricultural University)
(Chinese Academy of Agriculture Sciences)
Thi,
P.
D.
V.
, Linxi,
C.
, Yan,
Z.
, Hong,
X.
L.
, Hai,
Y.
B.
, &
Jianbo,
C.
(2017). Development of HPLC method for differentiation of three parts of mulberry tree. Analytical Science and Technology, 30(3), 130-137, https://doi.org/10.5806/AST.2017.30.3.130
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Abstract
The leaves (Mori Folium; MF), branches (Mori Ramulus; MR), and root bark (Mori Cortex Radicis; MCR) of the mulberry tree have been used as therapeutic herbs for centuries. Existing analytical methods were developed specifically for different parts of the tree and cannot be applied to samples containing a mixture of tree parts. Such method specialization is time-consuming and requires separate identification and quality control of each tree part. This report describes an HPLC method for the simultaneous quality control and discrimination of MF, MR, and MCR using four marker compounds: rutin, kuwanon G, oxyresveratrol, and morusin. An Optimapak C18 column (4.6 × 250 mm, 5 μm) was used with a gradient elution of 0.1 % formic acid in water and acetonitrile. The flow rate was 1.0 mL/min and the detection wavelength was 270 nm. In quantitative analyses of the three parts, rutin (0.11 % w/w) was detected only in MF. The oxyresveratrol content (0.12 % w/w) was highest in MR. Kuwanon G (0.33 % w/w) and morusin (0.18 % w/w) were higher in MCR than in other parts. The HPLC method given herein can be used to simultaneously classify and quantify three herbal medicines from the mulberry tree.
- keywords
- HPLC, mori folium, mori ramulus, mori cortex radicis, simultaneous analytical method
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