- P-ISSN 1225-0163
- E-ISSN 2288-8985
- Log In/Sign Up
- P-ISSN1225-0163
- E-ISSN2288-8985
- SCOPUS, ESCI, KCI
Article Detail
Home > Article Detail
Detection of phenobarbital adulteration in dietary supplements: simultaneous analysis of 16 sedative-hypnotics and sleep-inducers by ultra-high-performance liquid chromatography with UV detection (UPLC-UV) and quadruple Orbitrap mass spectrometry (Q-Orbitrap-MS)
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
2019, v.32 no.1, pp.24-34
https://doi.org/10.5806/AST.2019.32.1.24
(Division of Advanced Analysis, National Institute of Food and Drug Safety Evaluation, Ministry of Food &)
(Division of Advanced Analysis, National Institute of Food and Drug Safety Evaluation, Ministry of Food &)
(Division of Advanced Analysis, National Institute of Food and Drug Safety Evaluation, Ministry of Food &)
(Division of Advanced Analysis, National Institute of Food and Drug Safety Evaluation, Ministry of Food &)
(Division of Advanced Analysis, National Institute of Food and Drug Safety Evaluation, Ministry of Food &)
(Division of Advanced Analysis, National Institute of Food and Drug Safety Evaluation, Ministry of Food &)
(Division of Advanced Analysis, National Institute of Food and Drug Safety Evaluation, Ministry of Food &)
(Division of Advanced Analysis, National Institute of Food and Drug Safety Evaluation, Ministry of Food &)
(Division of Advanced Analysis, National Institute of Food and Drug Safety Evaluation, Ministry of Food &)
(Division of Advanced Analysis, National Institute of Food and Drug Safety Evaluation, Ministry of Food &)
(Division of Advanced Analysis, National Institute of Food and Drug Safety Evaluation, Ministry of Food &)
(Division of Advanced Analysis, National Institute of Food and Drug Safety Evaluation, Ministry of Food &)
(Division of Advanced Analysis, National Institute of Food and Drug Safety Evaluation, Ministry of Food &)
(Division of Advanced Analysis, National Institute of Food and Drug Safety Evaluation, Ministry of Food &)
(Division of Advanced Analysis, National Institute of Food and Drug Safety Evaluation, Ministry of Food &)
Ji,
H.
L.
, Ji,
Y.
C.
, Hanna,
P.
, Ah,
Y.
M.
, Nam,
S.
K.
, Seong,
S.
P.
, Sung-Kwan,
P.
, &
Ho-il,
K.
(2019). Detection of phenobarbital adulteration in dietary supplements: simultaneous analysis of 16 sedative-hypnotics and sleep-inducers by ultra-high-performance liquid chromatography with UV detection (UPLC-UV) and quadruple Orbitrap mass spectrometry (Q-Orbitrap-MS). Analytical Science and Technology, 32(1), 24-34, https://doi.org/10.5806/AST.2019.32.1.24
- Downloaded
- Viewed
Abstract
The safety of food is occasionally questionable, as there have been some reports of products contaminated with illegal adulterants. In this study, the presence of 16 sedative-hypnotics and sleep inducers in dietary supplements was determined by ultra-high-performance liquid chromatography with UV detection (UPLC-UV) and quadruple Orbitrap mass spectrometry (Q-Orbitrap-MS). The UPLC method was validated, providing a linearity (R2) of more than 0.999, and LODs and LOQs that ranged from 0.2 to 0.5 and 0.6 to 1.5 μg mL−1, respectively. The repeatabilities were 0.2-8.4 % (intra-day) and 0.3-4.5 % (inter-day), and the accuracies were 89.0-117.0 % (intra-day) and 87.8-111.9 % (inter-day). The mean recoveries of the spiked samples ranged from 98.7 to 107.3 %. The relative standard deviation (%RSD) of the stability was less than 2.4 %. Using the developed method, one sedative-hypnotic compound, phenobarbital, was detected in one of the nineteen samples tested. In addition, the major characteristic fragment ions of each target compound were confirmed using Q-Orbitrap-MS for higher accuracy. Monitoring the presence of these 16 sedative-hypnotics and sleep inducers in dietary supplements should be pursued in the interest of human health, and the results of this study confirmed that the developed method has value for this application.
- keywords
- Sedative-Hypnotics, Adulterants, UPLC-UV, Q-Orbitrap-MS, Method Validation
Reference
1
1. Q. Gao, B. Che, Z. Zhang, H. Dai, and H. Guo, J. Chin. Pharm. Univ., 39, 142-146 (2008).
2
2. B. Zhu, Z. Zhao, Z. Wang, Q. Xue, and L. Zhao, Chin. J. Pharm. Anal., 30, 745-751 (2010).
3
3. J. Liu, L. Xiao, X. Guan, Y. Xiong, T. Wang, and J. Li, Chin. J. Pharm. Anal., 31, 376-379 (2011).
4
4. P. Jin, Z. Wang, W. Wang, D. Zou, J. Ma, and X. Hu, Chin Pharm. J., 47, 462-466 (2012).
5
5. L. J. Greenfield, S. H. Zaman, M. L. Sutherland, S. C. Lummis, M. I. Niemeyer, E. A. Barnard, and R. L. Macdonald, Neuropharmacology, 42, 502-521 (2002).
6
6. C. Gottesmann, Neuroscience, 111, 231-239 (2002).
7
7. J. Wang, B. Chen, and S. Yao, Food addit. Contam., 25, 822-830 (2008).
8
8. Z. Wang, J. Zhang, and Y. Zhang, J. Chin. Mass Spec. Soc., 30, 282-286 (2009).
9
9. A. Yan, X. Li, C. Xi, L. Zhang, S. Xia, G. Wang, B. Tang, and Z. Mu, Chinese J. Anal. Chem., 41, 509-516(2013).
10
10. E. Deconinck, P. Y. Sacré, P. Courselle, and J. O. De Beer, J. Chromatogr. Sci., 51, 791-806 (2013).
11
11. AOAC Official Methods of Analysis. Internationalconference on harmonization of harmonized tripartiteguideline, validation of analytical procedures: text andmethodology.
12
12. AOAC International, Rockville, MD (2005).
13
13. ICH International conference on Harmonization. Harmonized tripartite guideline, validation of analyticalprocedures:text and methodology Q2
14
14. (R1). European Medicines Agency (EUMA), London, UK .
15
15. A, El-Gindy, Il Farmaco, 60, 745 (2005).
16
16. R. A. Menck, D. S. De Lima, S. C. Seulin, V. Leyton, C. A. Pasqualucci, D. R. Munoz, M. D. Osseltore, M. Yonamine, J. Sep. Sci., 35, 3361-3368 (2012).
17
17. X. Zhang, Z. Lin, J. Li, Z. Huang, Y. Rao, H. Liang, J. Yan, and F. Zheng. Drug Testing and Analysis, 9, 588-595 (2017).
Other articles from this issue
- Simultaneous uptake of arsenic and lead using Chinese brake ferns (Pteris vittata) with EDTA and electrodics
- Discrimination model for cultivation origin of paper mulberry bast fiber and Hanji based on NIR and MIR spectral data combined with PLS-DA
- Characterization of contribution of vehicle emissions to ambient NO2 using stable isotopes
- Detection of phenobarbital adulteration in dietary supplements: simultaneous analysis of 16 sedative-hypnotics and sleep-inducers by ultra-high-performance liquid chromatography with UV detection (UPLC-UV) and quadruple Orbitrap mass spectrometry (Q-Orbitrap-MS)
- more
Recommanded Articles
This website recommended the use the Chrome browser for journal website.