- P-ISSN 1225-0163
- E-ISSN 2288-8985
- P-ISSN1225-0163
- E-ISSN2288-8985
- SCOPUS, ESCI, KCI
Article Detail
Home > Article Detail
Determination of nadolol enantiomers in human plasma using a coupled achiral-chiral high-performance liquid chromatography method
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
2020, v.33 no.2, pp.59-67
https://doi.org/10.5806/AST.2020.33.2.59
(College of Pharmacy, Kangwon National University)
(College of Pharmacy, Kangwon National University)
(College of Pharmacy, Kangwon National University)
(College of Pharmacy, Kangwon National University)
(Pharmacy Faculty, Can Tho University of Medicine and Pharmacy, Vietnam)
(College of Pharmacy, Chungnam National University)
(Natural Products Research Institute, College of Pharmacy, Seoul National University)
(College of Pharmacy, Kangwon National University)
(College of Pharmacy, Kangwon National University)
(College of Pharmacy, Kangwon National University)
(College of Pharmacy, Kangwon National University)
(Pharmacy Faculty, Can Tho University of Medicine and Pharmacy, Vietnam)
(College of Pharmacy, Chungnam National University)
(Natural Products Research Institute, College of Pharmacy, Seoul National University)
(College of Pharmacy, Kangwon National University)
Seung-Beom,
L.
, Thuy-Vy,
P.
, Xuan-Lan,
M.
, Thi-Anh-Tuyet,
L.
, Thi-Ngoc-Van,
N.
, Jong-Seong,
K.
, Woongchon,
M.
, &
Kyeong,
H.
K.
(2020). Determination of nadolol enantiomers in human plasma using a coupled achiral-chiral high-performance liquid chromatography method. Analytical Science and Technology, 33(2), 59-67, https://doi.org/10.5806/AST.2020.33.2.59
- Downloaded
- Viewed
Abstract
Nadolol is a β-blocker drug, which effectively manages hypertension and angina pectoris. Its chemical structure allows the formation of four possible stereoisomers. A coupled column high-performance liquid chromatographic (HPLC) system with UV and fluorescence detection was investigated for simultaneously determining four nadolol enantiomers in human plasma. The plasma samples were prepared using a convenient liquid-liquid extraction process and passed through HPLC. Nadolol was initially separated from the endogenous compounds or other impurities in human plasma on a Phenomenex silica column, and its enantiomers were resolved and determined on a Chirapak AD-H column. The developed HPLC method achieved an effective chiral separation and significantly eliminated endogenous compound interference. This optimal HPLC method was validated following FDA guidelines. The results showed good selectivity, linearity, accuracy (90.50 % – 105.27 %), and precision (RSDs < 9.52 %) for each enantiomer. This method was also successfully applied to determine nadolol enantiomers in the plasma samples of a healthy male volunteer (after orally administering 80 mg racemic nadolol), proving its suitability for nadolol stereoselective pharmacokinetic studies.
- keywords
- Nadolol, <TEX>${\beta}$</TEX>-blocker, stereoselective analysis, coupled achiral-chiral HPLC, pharmacokinetic study
Reference
1
1. X. Wang and C. B. Ching, J. Chromatogr. A, 1035, 167-176 (2004).
2
2. R. Cižmáriková, L. Habala, J. Valentová and M. Markuliak, Appl. Sci., 9, 625 (2019).
3
3. B. S. Sekhon, Int. J. Pharmtech. Res., 2, 1584-1594 (2010).
4
4. X. Wang and C. B. Ching, Chirality, 14, 798-805 (2002).
5
5. X. Wang and C. B. Ching, J. Sep. Sci., 30, 333-343(2007).
6
6. W. Bragg, D. Norton and S. A. Shamsi, J. Chromatogr. B Analyt. Technol. Biomed. Life Sci., 875, 304-316(2008).
7
7. B. Francesco, C. Carmen, G. Lucia and L. R. M. Immacolata, Eur. J. Pharm. Sci., 38, 472-478 (2009).
8
8. A. E. Ribeiro, A. E. Rodrigues and L. S. Pais, Chirality, 25, 197-205 (2013).
9
9. Z. A. Al-Othmana, A. Al-Warthana, S. D. Alamb and I. Ali, Biomed. Chromatogr., 28, 1514-1524 (2014).
10
10. I. Ali, M. Suhail, M. N. Lone, Z. A. Alothman and A. Alwarthan, J. Liq. Chrom. Relat. Tech., 39, 435-444(2016).
11
11. J. B. Frank, A. P. Mark, R. S. Nuggchally, H. B. Rashmi and A. B. Ian, Biomed. Chromatogr., 9, 140-145 (1995).
12
12. H. Masanori, Y. Koichi, S. Yasutaka and O. Akira, J. Chromatogr. B, 661, 281-289 (1994).
13
13. I. W. Wainer, J. Pharm. Biomed. Anal., 7, 1033-1038(1989).
14
14. K. H. Kim, K. J. Kim, J. S. Kang and W. Mar, J. Pharm. Biomed. Anal., 22, 377-384 (2000).
15
15. K. H. Kim, H. W. Yun, H. J. Kim, H. J. Park and P. W. Choi, Arch. Pharm. Res., 21, 212-216 (1998).
16
16. K. H. Kim, H. J. Kim, J. H. Kim and S. D. Shin, J. Chromatogr. B, 751, 69-77 (2001).
17
17. J. C. Barreiro, K. L. Vanzolini and Q. B. Cass, J. Chromatogr. A, 1218, 2865-2870 (2011).
18
18. Z. Deáková, Z. Ďuračková, D. W. Armstrong and J. Lehotay, J. Liq. Chrom. Relat. Tech., 38, 789-794 (2015).
19
19. N. C. B. Albuquerque, J. V. Matos and A. R. M. Oliveira, J. Chromatogr. A, 1467, 326-334 (2016).
Other articles from this issue
- Determination of nadolol enantiomers in human plasma using a coupled achiral-chiral high-performance liquid chromatography method
- Development of nitrogen and oxygen certified reference materials in 10 μmol/mol for the purity evaluation
- Electrochemically polyaniline-coated microextraction needle for phthalates in water
- Study on the spectroscopic reconstruction of explosive-contaminated overlapping fingerprints using the laser-induced plasma emissions
- Impurity profiling and chemometric analysis of methamphetamine seizures in Korea
- Latent fingerprint development from rubber gloves using MMD I (Multimetal deposition I)
- more
Recommanded Articles
This website recommended the use the Chrome browser for journal website.