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    Vol.34, No.5
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    Method development and validation of spectrophotometric and RP-HPLC methods for simultaneous estimation of spironolactone and furosemide in bulk and combined tablet dosage forms

    Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
    2021, v.34 no.5, pp.212-224
    https://doi.org/10.5806/AST.2021.34.5.212
    Rohankumar R. Chavan (Dept. of Pharmaceutical Chemistry, Bharati Vidyapeet College of Pharmacy)
    Somnath D. Bhinge (Dept. of Pharmaceutical Chemistry, Rajarambapu College of Pharmacy)
    Mangesh A. Bhutkar (Dept. of Pharmaceutics, Rajarambapu College of Pharmacy)
    Dheeraj S. Randive (Dept. of Pharmaceutics, Rajarambapu College of Pharmacy)
    Vijay R. Salunkhe (Dept. of Pharmacognosy, Rajarambapu College of Pharmacy)
    Rohankumar, R. C. , Somnath, D. B. , Mangesh, A. B. , Dheeraj, S. R. , & Vijay, R. S. (2021). Method development and validation of spectrophotometric and RP-HPLC methods for simultaneous estimation of spironolactone and furosemide in bulk and combined tablet dosage forms. Analytical Science and Technology, 34(5), 212-224, https://doi.org/10.5806/AST.2021.34.5.212
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    Abstract

    The intent of the present work was to develop a simple, sensitive, accurate, precise, rapid and economical UV- spectrophotometric and reverse phase high pressure liquid chromatographic method for the simultaneous estimation of Spironolactone and Furosemide in bulk and combined tablet dosage forms. UVSpectrophotometry was carried out by simultaneous equation method using 0.02 M potassium dihydrogen phosphate buffer pH 3.5: Acetonitrile (50:50) v/v as a solvent. The linearity range was 2-14 μg mL−1 for Spironolactone and Furosemide with a correlation coefficient > 0.99. The chromatographic separation was achieved on 250 mm × 4.6 mm, hypersil BDS C18 column with particle size 5 μm, by using an isocratic mixture of 0.02 M potassium dihydrogen phosphate buffer pH 3.5: Acetonitrile: tert butyl methyl ether (49:50:1) v/v/v as a solvent at a flow rate of 1 mL min−1 and UV detection was carried out at 254 nm. The retention time were observed to be 3.666 and 6.661 minutes for Furosemide and Spironolactone respectively. The two developed methods were validated according to the ICH guidelines for accuracy, precision, linearity, LOD, LOQ and were found to be within the limits. It can be concluded that these two methods could be successfully used for the simultaneous estimation of Spironolactone and Furosemide in bulk and combined tablet dosage forms.

    keywords
    UV-spectroscopic, RP-HPLC, spironolactone, furosemide


    Reference

    1

    1. G Giebisch, Arzneimittelforschung, 35(1A), 336-342(1985).

    2

    2. A. Qureshi and I. J. McGilveray, Drug Dev. Ind. Pharm., 24, 995-1005 (1998).

    3

    3. M. E. Bosch, A. J. R. Sánchez, F. S Rojas and C. B. Ojeda, Int. J. Pharm. Biol. Sci., 3, 168-181 (2013).

    4

    4. E. Dinç and Ö. Üstünda?, Farmaco, 58, 1151-1161(2003).

    5

    5. G. Kher, V. Ram, M. Kher and H. Joshi, Res. J. Pharm. Biol. Chem. Sci., 4, 365-377 (2013).

    6

    6. J. N. Tandel, Eura. J. Anal. Chem., 12, 385-394 (2017).

    7

    7. https://liverfellow.org/post/diuretics-ascites, Assessed 24July 2021.

    8

    8. I. Velat, Ž. Bušić, M. J. Paić and V. Čulić, BMC Pharmacol. Toxicol., 21, 57 (2020).

    9

    9. H. Patel and S. Solanki, Asian J. Pharm. Clin. Res., 5, 195-198 (2012).

    10

    10. S. Naveed, F. Qamar and S. Zainab, J. Innov. Pharm. Biol. Sci., 1, 97-101 (2014).

    11

    11. M. C. F. Ferraro, P. M. Castellano and T. S. Kaufman, J. Pharm. Biomed. Anal., 34, 305-314 (2004).

    12

    12. M. L. Luis, J. M. García, F. Jiménez, A. J. Jiménez and J. J. Arias, J. AOAC Int., 82, 1054-1063 (1999).

    13

    13. H. Patel and S. Solanki, Int. J. Pharm. Pharm. Sci., 4, 2-5 (2012).

    14

    14. M. A. Hegazy, F. H. Metwaly, M. Abdelkawy and N. S Abdelwahab, J. Chromatogr. Sci., 49, 129-135 (2011).

    15

    15. M. C. Ferraro, P. M. Castellano and T. S. Kaufman, J. Pharm. Biomed. Anal., 26, 443-451 (2001).

    16

    16. S. Chawla, S. Ghosh, V. Sihorkar, R. Nellore, T. R. S. Kumar and N. R. Srinivas, Biomed. Chromatogr., 20, 349-357 (2006).

    17

    17. N. Dubey, G. Patil, D. Kumar and J. Subhash, Int. J. Biomed. Pharm. Sci., 7, 38-41 (2013).

    18

    18. I. Youm and B. C. Youan, J. Anal. Meth. Chem., 2013, Article ID 207028 (2013).

    19

    19. R. Laxman, A. Ashish, J. Vinay, B. Sudhir and J. Deepti, Int. J. Res. Ayurveda Pharm., 1, 459-467 (2010).

    20

    20. J. M. Sandall, J. S. Millership, P. S. Collier and J. C. McElnay, J. Chromatogr. B Anal. Tech. Biomed. Life Sci., 839, 36-44 (2006).

    21

    21. S. S. Israt, M. N. Uddin, R. A. Jahan and M. M. Karim, Bangladesh J. Sci. Indust. Res., 51, 297-306 (2016).

    22

    22. M. Bhojani, K. Dadhania and S. Faldu, Journal of Pharmaceutical Science and Bioscientific Research, 2, 144-147 (2012).

    23

    23. A. S. S. V. Reddy, M. Ahmed and A. S. K. Ashetty, Int. J. ChemTech Res., 5, 1876-1885 (2013).

    24

    24. P. Parimoo, A. Bharathi and K. Padma, Indian J. Pharm. Sci., 57(3), 126-129 (1995).

    25

    25. J. Thriveni, R. Rambabu, J. V Rao and S. Vidyadhara, Int. J. Res. Pharm. Chem., 3, 208-212 (2013).

    26

    26. M. Bhojani, K. Ketan and F. Shital, J. Pharm. Sci. Biosci. Res., 2, 144-147 (2012).

    27

    27. V. R. Ram, P. N. Dave and H. S. Joshi, J. Chromatogr. Sci., 50, 721-726 (2012).

    28

    28. C. Vadloori and V. Tallada, J. Pharm. Res., 5(8), 3998-4000 (2012).

    29

    29. Q2A ICH guideline, Validation of analytical procedures text methodology, 2003.

    30

    30. S. D. Bhinge, S. M. Malipatil and L. V. Sonawane, Thai J. Pharm. Sci., 36, 63-71 (2012).

    31

    31. The United States Pharmacopoeia 35 National formulary

    32

    30. Eas-ton; R and Mc Nally Taunton; (2012).

    33

    32. Y. D. Dange, V. R. Salunkhe, S. D. Bhinge, B. R. Bhutkar and Y. H. Momin, Indian Drugs, 55, 61-66, (2017).

    34

    33. Y. D. Dange, S. M. Honmane, S. D. Bhinge, V. R. Salunkhe and D. R. Jadge, Indian J. Pharm. Edu. Res., 52, S46-S52 (2018).

    35

    34. S. D. Bhinge, S. M. Malipatil and L. V. Sonawane, Acta Chim. Slov., 61, 580-586 (2014).

    36

    35. S. D. Bhinge, S. M. Malipatil, A. Jondhale, R. Hirave and A. S. Savali, E-J. Chem., 9, 1223-1229 (2012).

    37

    36. S. D. Bhinge, S. M. Malipatil, A. Jondhale and A. S. Savali, Asian J. Chem., 25, 981-985 (2013).

    38

    37. http://ccc.chem.pitt.edu/wipf/Web/LCMS%20trouble%20shooting.pdf, Accessed on 11/06/2021.

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