- P-ISSN 1225-0163
- E-ISSN 2288-8985
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Determination of Urea using Rose Tissue Sensor
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
1993, v.6 no.3, pp.313-318
Kim,
B., Jeon,
Y., &
Chung,
C.
(1993). Determination of Urea using Rose Tissue Sensor. Analytical Science and Technology, 6(3), 313-318.
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Abstract
The rose petal tissue biosensor has been constructed by immobilizing New carina rose tissue. Optimum conditions for the determination of urea were investigated using this sensor. Selectivity and life time of this sensor were also obtained. As a result, the biosensor showed the optimum response characteristics in 0.20M phosphate buffer solution at pH 8.0, <TEX>$37^{\circ}C$</TEX> and 50mg of tissue amounts. This sensor was linear from <TEX>$9.0{\times}10^{-5}$</TEX> to <TEX>$4.0{\times}10^{-3}M$</TEX> urea with a slope of 42mV/decade. The limit of detection and response time are <TEX>$7.0{\times}10^{-5}M$</TEX> and 17~19 min.
- keywords
- Rose petal biosensor, Urea
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