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Structural Determination of Cerebrosides from Soybean Embryo by Mass Spectrometer
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
1993, v.6 no.3, pp.335-343
Kim,
J., Chang,
S., &
Kim,
Y.
(1993). Structural Determination of Cerebrosides from Soybean Embryo by Mass Spectrometer. Analytical Science and Technology, 6(3), 335-343.
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Abstract
The structure of cerebrosides from soybean embryo was determined using fast atom bombardment mass spectrometer (FAB-MS), gas chromatography mass spectrometer (GC-MS) and TLC. The components of cerebroside were determined by GC-MS after acid hydrolysis. The molecular weight distribution of cerebroside was measured by positive mode FAB-MS with LiOH saturated 3-nitrobenzylalcohol(3-NBA) matrix. Structures of individual components of complex mixtures can be determined easily by this process. The major constituent of soybean extracted cerebroside was determined as the glucoside of N-2'-hydroxypalmitoyl-sphingadienine.
- keywords
- Cerebroside, Soybean Embryo, FAB-MS, GC-MS
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