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- E-ISSN 2288-8985
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A Study on the Separation of Racemic Amino acids in Food or Biological Sample with GLC
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
1994, v.7 no.1, pp.53-64
Rhee,
J., Eo,
Y., Park,
H., &
Kim,
T.
(1994). A Study on the Separation of Racemic Amino acids in Food or Biological Sample with GLC. Analytical Science and Technology, 7(1), 53-64.
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Abstract
After establishment of methodology for the separation and quantitation of optical isomer existed in food or biomaterial, the relationship between isomer and nutrient or biological sample was investigated. The optical isomers of standard amino acids and free amino acids were quantitized and the protein was assayed from the Korean bean, pasted bean, soy sauce, gochujang, powderd milk and cataract followed by hydrolysis and dervatization with TFA-IPA for GLC analysis with chirasil val column. Amino acids showing the racemization were alanine, aspartic acid, glutamic acid and phenyl alanine. The most convenient amino acid deducing age and biological activity was aspartic acid. Glutamic acid and phenyl alanine have shown poor resolution with less racemization. The ratio of d-form amino acids was 3~6% for home made pasted bean, about 3% for commerical pasted bean, 2~4% for soy sauce, about 1% for bean, 1~2% for cataract, 1.0~1.5% for powdered milk. The racemization during fermentation process was significant.
- keywords
- Amino acids, optical isomer, TFA-IPA, GLC, chirasil val column
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