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- E-ISSN 2288-8985
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- P-ISSN1225-0163
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Application of Hepatocyte Specific Polymers with Functional group
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
1996, v.9 no.1, pp.84-90
Lee,
J. B., &
Kim,
J.
W.
(1996). Application of Hepatocyte Specific Polymers with Functional group. Analytical Science and Technology, 9(1), 84-90.
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Abstract
New hepatocyte specific copolymers, that have oligosaccharide and biotin residue on the side chain of styrene, were designed and synthesized to use as a multifunctional recognition. In order to measure initial adhesion efficiency, 1mL of copolymer solutions (0.01%, w/v) such as p(VLA-co-VBA) 90 : 10, p(VLA-co-VBA) 80 : 10 and PYLA as a standard were added to polystyrene petri dish, respectively. In the absence and presence of serum, hepatocyte solution of rat by method of Seglen was added. After 60 min, adhesion efficiency was 70%, that is similar to those of the absence of serum. Aggregation capacity between biotin residue in p(VLA-co-VBA) 70 : 30 and avidin was measured by using UV-transmittance.
- keywords
- Oligosaccharide, Biotin, Copolymer, Hepatocyte, VLA
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