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Characteristics of Heterotrophic Bacterial Population in the Artificial Lake Geumgang Near Estuary Barrage

Journal of Ecology and Environment / Journal of Ecology and Environment, (P)2287-8327; (E)2288-1220
2005, v.28 no.3, pp.129-134





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Abstract

The monthly variations of physico-chemical and microbiological water quality were investigate in the artificial Lake Geumgang near estuary barrage. Sixty heterotrophic bacteria were isolated and identified by amplification and sequencing of 16S rDNA. Water temperature, pH, and inorganic nutrients(NH4-N, NO2-N, NO3-N, PO4-P) were measured. Concentrations of DO, BOD, and inorganic nutrients were lower than in the middle-stream of Geum river. The population densities of heterotrophic bacteria and total coliforms varied from 4.1 ± 1.0 × 102 to 6.7 ± 1.1 × 103 cfu ml-1, and 0 to 2.3 ± 0.6 × 102 cfu ml-1, respectively. Among the measured numbers of physiological groups of bacteria, cellulolytic bacteria showed higher population densities than those of other physiological groups. Bacterial community structure was analysed based on 16S rDNA partial sequencing. Among 60 isolates, dominant genus was seudomonas (20 strains).

keywords
Artificial Lake Geumgang, Heterotrophic bacteria, Physico-chemical water quality, Physiological groups of bacteria, Total coliform, 16S rDNA analysis

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Journal of Ecology and Environment