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  • KOREAN
  • P-ISSN2287-8327
  • E-ISSN2288-1220
  • SCOPUS, KCI

Changes of Thiols and Oxidative Stress in Tomato Seedlings Exposed to Cadmium

Journal of Ecology and Environment / Journal of Ecology and Environment, (P)2287-8327; (E)2288-1220
2006, v.29 no.1, pp.61-67


Abstract

Tomato (Lycopersicon esculentum Mill) seedlings exposed to various concentrations of CdCl2 (0∼100 μM) in a nutrient solution for up to 9 days were analyzed with respect to the thiol changes and oxidative stress. The Cd exposure increased total non-protein thiols (NPT) and cysteine in both leaves and roots, total glutathione in leaves, and the ratios of oxidized glutathione (GSG)/reduced glutathione (GSH) in both leaves and roots, but decreased the ratio of dehydroascorbate (DASA)/ascorbate(ASA) in leaves. Our results sugest that the Cd-induced GSH depletion due to thiol synthesis and oxidation alters the antioxidant activity of seedlings for H2O2 2O2 accumulationand oxidative stress result in phytotoxicity.

keywords
Cadmium, Oxidative stress, Thiols, TomatoUn-Haing Cho and Nam-Ho Seo J. Ecol. Field Biol. 29 (1) 62induces ROS production and oxidative stress is also an interesting subject to investigate.In this work, the changes of NPTs and ascorbate in tomato se, Cadmium, Oxidative stress, Thiols, TomatoUn-Haing Cho and Nam-Ho Seo J. Ecol. Field Biol. 29 (1) 62induces ROS production and oxidative stress is also an interesting subject to investigate.In this work, the changes of NPTs and ascorbate in tomato se

Reference

1.

(1989) Biosynthesis and antioxidant function of glutathionein plants,

2.

(1992) Ascorbate peroxidase ?C A hydrogen peroxide scavengingenzyme in plants,

3.

(1990) Plant water relations as affected byheavy metal stress,

4.

(1990196-204) ㈍-glutamylcysteine and glutathione contents of spinach leaves asaffected by darkness and application of excess sulfur Glutathioneaccumulation in detached leaves exposed to H2S in theabsence of light is stimulated by the supply of glycine to thepetiole,

5.

(2000) Mercury-induced oxidative stress in tomato seedlings,

6.

(2004) Cadmium-induced phytotoxicity in tomato seedlings due to the accumulation of H2O2 that results from the reduced activities of H2O2 detoxifying enzymes.,

7.

(2005) Oxidative stress in Arabidopsis thalianaexposed to cadmium is due to hydrogen peroxide accumulation,

8.

(1997) Candmiumvand zinc induction of lipid peroxidation and effects on antioxidantvenzyme activities in bean(Phaseolus vulgaris L.). ,

9.

(1991) Increased resistance to copper-induced damage of the root cellplasmalemma in copper-tolerant Silene cucubalus,

10.

(1992) Glutathione depletiondue to copper-induced phytochelatin synthesis causes oxidativestress in Sulene cucubalus,

11.

(1993) Effect of copper on fatty acid composition and peroxidation oflipids in the roots of copper-tolerant and?Csensitive Silene cucubalus,

12.

(1989) glycine synthesis in Datura innoxia and bindingwith cadmium,

13.

(2001) Differential antioxidative responses to cadmium in roots and leaves of pea (Pisum sativum L. cv.Azad). ,

14.

(2002) Phytochelatin synthesis isnot responsible for Cd tolerance in the Zn/Cd hyperaccumulatiorThlapsi ,

15.

(2000) Combinationtoxicology of metal-enriched soils physiological responses of aZn- and Cd-resistant ecotype of Silene vulgaris on polymetallicvsols,

16.

(1997) Salinity, oxidative stress and antioxidant responses in shoot cultures of rice,

17.

(1967) A spectrophotometric method for the directdetermination of cysteine in the presence of other naturallyoccurring amino acids,

18.

(1985) the principalheavy-metal complexing peptites of higher plants,

19.

(1999) Phytochelatin synthase genes from Arabidopsis and the yeast Schizosaccharomyces pombe,

20.

(2001) Comparative studies of H2O2detoxifying enzymes in green and greening barley seedlings undercadmium stress,

21.

(1938) The water culture method for growingplants without soil,

22.

(1987) its role in cadmium reistance inplant cells,

23.

(1995) The mechanism of the stimulation of state 4 respirartion by cadmium in potato tuber(Solanum tuberosum) mitochondria.,

24.

(2004) Tissue- and age-dependent differences in the complexation of cadmium and zinc in the cadmium/zinc hyperaccumulator Thlaspicaerulescens revealed by X-ray absorption spectroscopy,

25.

(2003) Overexpression of Arabidopsis phytochelatin synthase paradoxicallyleads to hypersensitivity to cadmium stress,

26.

(2000) Phytochelatins and metal tolerance. In : Environmental Pollution and Plant Responses , CRC Press

27.

(1996) Synthesis of glutathione in leaves of transgenic poplar Populus tremula √ P. alba) overexpressing ㈍-glutamylcysteine synthetase.,

28.

(1998) Keeping activeoxygen: Keeping active oxygen under control.,

29.

(1998a) Glutathione: Biosynthesis, metabolism and relationship to stress tolerance explored in transformed plants. ,

30.

(1998b) Manipulation of glutathione and amino acid biosynthesis in the chloroplast,

31.

(1997) Cadmium- and copper-induced changes in tomato membrane lipids,

32.

(2000) Overexpression ofglutathione reductase in Brassica juncea effects on cadmiumaccumulation and tolerance,

33.

(1987) Changes in glutathione content of maize seedlingsexposed to cadmium,

34.

(1995) Phytochelatins and related peptides: Structure,biosynthesis, and function.,

35.

(1992) Effect of cadmium on ㈍-glutamylcysteinesynthesis in maize seedlings,

36.

(1989) Copperphytochelatins of Mimulus guttatus, Salt DE

37.

(1999) Responses to cadmium in higher plants,

38.

(2002) The role of phytovhelatins in constitutive and adaptative heavymetal tolerances in hyperaccumulator and non-hyperaccumulator metallophytes,

39.

(1987) Phytochelatinsynthesis and glutathione levels in response to heavy metals intomato cells,

40.

(1995) Regulation of glutathione synthesis in suspension cultures of parsley and tobacco,

41.

(2001) Cadmium-induced changes in antioxodative systems hydrogen pero-February 2006 Thiols and Oxidative Stress in Tomato 67xide content and differentiation in Scots Pine roots,

42.

(1993199-202) Inhibition of electron flow aroundphotosystem I in chloroplasts of Cd-treated maize plants is due toCd-induced iron deficiency,

43.

(1986) Accumulation of non-protein metal-binding polypeptides,

44.

(1990) Changes in glutathione and phytochelatinsin roots of maize seedlings exposed to cadmium,

45.

(1985) Effects of heavymetal stress on the genetic structure of populations of Silenecucubalus Structure and Functioning of Plant Populations,

46.

(1996) Relationship between cadmium glutathione and cadmium-binding peptides (phytochelatins) in leaves of intact tobacco seedlings,

47.

(1997) Zn phytotoxicity induces oxidativestress in primary leaves of Phaseolus vulgaris,

48.

(1987) evidencefor the role of singlet oxygen and endogenous antioxidants,

49.

(2001) The biologicalfunctions of glutathione revisited in Arabidopsis transgenic plantswith altered glutathione levels,

50.

(1999) Overexpressionof glutathione synthetase in Indian mustard enhances cadmiumaccumulation and tolerance,

Journal of Ecology and Environment