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Over-expression of Cu/ZnSOD Increases Cadmium Tolerance in Arabidopsis thaliana

Journal of Ecology and Environment / Journal of Ecology and Environment, (P)2287-8327; (E)2288-1220
2007, v.30 no.3, pp.257-264

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Abstract

Over-expresion of a coper/zinc superoxide dismutase (Cu /ZnSOD) resulted in substantialyincreased tolerance to cadmium exposure in Arabidopsis thaliana . Lower lipid peroxidation and H 2O2 acu-mulation and the higher activities of H2O2 scavenging enzymes, including catalase (CAT) and ascorbateperoxidase (APX) in transformants (CuZnSOD-tr) compared to untransformed controls (wt) indicated thatoxidative stres was the key factor in cadmium tolerance. Although progresive reductions in the dark-adaptedphotochemical eficiency (Fv/Fm) and quantum eficiency ield were observed with increasing cadmium levels,the chlorophyl fluorescence parameters were les marked in CuZnSOD-tr than in wt. These observationsindicate that oxidative stres in the photosynthetic aparatus is a principal cause of Cd-induced phytotoxicity,and that Cu/ZnSOD plays a critical role in protection against Cd-induced oxidative stres.

keywords
Arabidopsis, Cadmium tolerance, Oxidative stres, Superoxide dismutase.Cho, Un-Haing J. Ecol. Field Biol. 30 (3) 258at concentrations exceding their detoxification capacity, the levelsof superoxide radicals are levated and H2O2 acumulates due to ani, Arabidopsis, Cadmium tolerance, Oxidative stres, Superoxide dismutase.Cho, Un-Haing J. Ecol. Field Biol. 30 (3) 258at concentrations exceding their detoxification capacity, the levelsof superoxide radicals are levated and H2O2 acumulates due to ani

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(2007) Received July 9 Accepted August 2,

Journal of Ecology and Environment