ISSN : 2287-8327
A simple and eficient protocol was developed for culturingCu-tolerant and Cu-acumulating plantsvia pre-adaptation to Cu during plant isue culture. We ind uced multiple shots from begonia ( Begoniaevansiana Andr.) leaf explants on MS medium suplemented with naphtaieneacetic acid and benzyladenine.After 3 months, smal plantlets were transfered to MS medium suplemented with 10 . M CuCl 2 for pre-adap-tation to Cu and cultured for 5 months. Then, these plantletswere individualy planted in pots containing artificialsoil. An aditional 50 mg of Cu disolved in 1/4 strength MS s olution was aplied to each pot during irigationover the course of 2 months. We planted pre-adapted and control begonias in soil from the Il-Kwang Mine, anabandoned Cu mine in Pusan, Korea, to examine their ability to tolerate and acumulate Cu for phytore-mediation. Pre-adapted begonias acumulated 1,20 . g Cu/g dry rot isue over the course of 45 days. Onthe other hand, non-Cu-adapted controls acumulated only 85 . g Cu/g dry rot isue. To enhance Cuextraction, chelating agents, ethylenediamine tetracetic acid (EDTA)-dipotasiun and pyridine-2,6-dicarboxylicacid (PDA), were aplied. While the chelating agents did notenhance acumulation of Cu in the rots of controlbegonias, EDTA aplication increased the level of Cu in the rots of pre-adapted begonias twofold (to 2,50. g Cu/g dry rot isue). Because pre-adapted begonias acum ulated a large amount of Cu, mainly in theirrots, they could be used for phytostabilization of Cu-contaminated soils. In adition, as a flowering plant,begonias can be used to create aestheticaly pleasing remed iation sites.
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(2007) Received July 27 Accepted August 16,