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ISSN : 2287-8327

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Vol.34 No.3
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Effects of an aqueous red pine (Pinus densiflora) needle extract on growth and physiological characteristics of soybean (Glycine max)

Journal of Ecology and Environment / Journal of Ecology and Environment, (P)2287-8327; (E)2288-1220
2011, v.34 no.3, pp.279-286



& (2011). Effects of an aqueous red pine (Pinus densiflora) needle extract on growth and physiological characteristics of soybean (Glycine max). Journal of Ecology and Environment, 34(3), 279-286.
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Abstract

The effect of allelochemicals on growth, root nodule nitrogen fixation activity, and ion patterns of soybeans were investigated. We prepared 50 g/L (T50), 100 g/L (T100), and 200 g/L (T200) extract concentrations by soaking fresh red pine needles in a nutrient solution. Adding needles to the nutrient solution increased the content of total phenolic acids,osmolality, and total ions. The total phenolic content in the T50, T100, and T200 extracts were 206 ± 12.61, 335 ± 24.16,and 603 ± 12.30 mg gallic acid equivalents, respectively. The K^+, Mg^(2+), Ca^(2+), and PO_4^(3-) content increased by adding needles to the nutrient solutions, whereas SO_4^(2-) content decreased. The growth inhibition of soybeans was proportional to the needle extract concentrations, and the T100 and T200 concentrations resulted in remarkable growth inhibition. On day 20 after treatment, dry weight and nitrogen fixation activity of the root nodules were reduced by the T100 and T200 treatments,whereas the T50 treatment was not difference from the control. After day 10, total ion content in all treatment groups was not different in comparison with the control. However, total ionic content in all treatment groups decreased significantly compared with that in the control after day 20. The lowest total ion value was found for the T200 concentration. The T200 treatment also resulted in significantly reduced SO_4^(2-) content. The amounts of Mg^(2+), Ca^(2+), and Mn^(2+) were higher than those of the control for the T50 treatment on day 10 and for T100 on day 20 after treatment. A significant increase in osmolality was observed in the T200 treatment on day 10 and in the T100 treatment on day 20. These results suggest that under severe allelochemical stress conditions, a remarkable reduction in nodule formation, nitrogen fixation activity, and ion uptake eventually resulted in a decrease in leaf production. Furthermore, increased K+, Mg2+, Ca2+,Mn2+, and osmolality in soybeans exposed to lower concentrations of allelochemicals than the critical stress level helped overcome the stress.

keywords
allelophathy, mineral uptake, nitrogen fixation activity, osmolality, red pine, soybean

Reference

1.

Alsaadawi IS, Al-Hadithy SM, Arif MB. 1986. Effects of three phenolic acids on chlorophyll content and ions uptake in cowpea seedlings. J Chem Ecol 12: 221-227.

2.

Appel HM. 1993. Phenolics in ecological interactions: the importance of oxidation. J Chem Ecol 19: 1521-1552.

3.

Appleby CA. 1984. Leghemoglobin and Rhizobium respiration. Ann Rev Plant Physiol 35: 443-478.

4.

Barkosky RR, Einhellig FA. 1993. Effects of salicylic acid on plant-water relationships. J Chem Ecol 19: 237-247.

5.

Batish DR, Lavanya K, Singh HP, Kohli RK. 2007. Phenolic allelochemicals released by Chenopodium murale affect the growth, nodulation and macromolecule content in chickpea and pea. Plant Growth Regul 51: 119-128.

6.

Baziramakenga R, Leroux GD, Simard RR. 1995. Effects of benzoic and cinnamic acids on membrane permeability of soybean roots. J Chem Ecol 21: 1271-1285.

7.

Baziramakenga R, Simard RR, Leroux GD. 1994. Effects of benzoic and cinnamic acids on growth, mineral composition and chlorophyll content of soybean. J Chem Ecol 20: 2821-2833.

8.

Bell DT. 1974. The influence of osmotic pressure in tests for allelopathy. Trans Ill State Acad Sci 67: 312-317.

9.

Bergmark CL, Jackson WA, Volk RJ, Blum U. 1992. Differential inhibition by ferulic acid of nitrate and ammonium uptake in Zea mays L. Plant Physiol 98: 639-645.

10.

Blum U, Gerig TM. 2005. Relationships between phenolic acid concentrations, transpiration, water utilization, leaf area expansion, and uptake of phenolic acids: nutrient culture studies. J Chem Ecol 31: 1907-1932.

11.

Blum U, Rice EL. 1969. Inhibition of symbiotic nitrogen-fixation by gallic and tannic acid, and possible roles in old-field succession. Bull Torrey Bot Club 96: 531-544.

12.

Blum U, Shafer SR, Lehman ME. 1999. Evidence for inhibitory allelopathic interactions involving phenolic acids in field soils: concepts vs. an experimental model. Crit Rev Plant Sci 18: 673-693.

13.

Einhellig FA. 1996. Interactions involving allelopathy in cropping systems. Agron J 88: 886-893.

14.

Fishbeck K, Evans HJ, Boersma LL. 1973. Measurement of nitrogenase activity of intact legume symbionts in situ using the acetylene reduction assay. Agron J 65: 429-432.

15.

Fernandez C, Lelolng B, Vila B, Mévy JP, Robles C, Greff S, Dupouyet S, Bousquet-Mélou A. 2006. Potential allelopathic effect of Pinus halepensis in the secondary succession: an experimental approach. Chemoecology 16: 97-105.

16.

Inderjit, Stribig JC, Olofsdotter M. 2002. Joint action of phenolic acid mixtures and its significance in allelopathy research. Physiol Plant 114: 422-428.

17.

Kato-Noguchi H, Fushimi Y, Shigemori H. 2009. An allelopathic substance in red pine needles (Pinus densiflroa). J Plant Physiol 166: 442-446.

18.

Kil BS. 1989. Phytochemical Ecology: Allelochemicals, Mycotoxins and Insect Pheromones and Allomones. Institute of Botany, Academica Sinica Monograph Series No. 9, Taipei.

19.

Lee IK, Monsi M. 1963. Ecological studies on Pinus densiflora forest. I. Effects of plant substances on the floristic composition of the undergrowth. Bot Mag 76: 400-413.

20.

Lodhi MAK, Killingbeck KT. 1982. Effects of pine-produced chemicals on selected understory species in a Pinus ponderosa community. J Chem Ecol 8: 275-283.

21.

Lyu SW, Blum U. 1990. Effects of ferulic acid, an allelopathic compounds, on net P, K, and water uptake by cucumber seedlings in a split-root sysem. J Chem Ecol 16: 2429-2439.

22.

Morgan JM. 1984. Osmoregulation and water stress in higher plants. Annu Rev Plant Physiol 35: 299-319.

23.

Munns R. 2002. Comparative physiology of salt and water stress. Plant Cell Environ 25: 239-250.

24.

O’hara GW, Boonkerd N, Dilworth MJ. 1988. Mineral constraints to nitrogen fixation. Plant Soil 108: 93-110.

25.

Rice EL. 1984. Allelopathy. Academic Press, New York.

26.

Rice EL, Lin CY, Huang CY. 1981. Effects of decomposing rice straw on growth of and nitrogen fixation by Rhizobium. J Chem Ecol 7: 333-344.

27.

Singleton VL, Rossi JA Jr. 1965. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am J Enol Vitic 16: 144-158.

28.

Takahama U, Oniki T. 1997. A peroxidase/phenolics/ascorbate system can scavenge hydrogen peroxide in plant cells. Physiol Plant 101: 845-852.

29.

Walsh KB. 1995. Physiology of the legume nodule and its response to stress. Soil Biol Biochem 27: 637-655.

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