ISSN : 2287-8327
A study on the presence of benthic dinoflagellates in the intertidal zone along the coasts of Jeju Island, Korea was conducted during 2011 and 2012. Identification and taxonomic observations were made of the benthic dinoflagellate samples using light and epifluorescence microscopy. Thirty-seven dinoflagellate taxa belong to five orders, nine families, 18genera and 30 species, which are new records for Korean waters, were detected in this study. The detailed nomenclature,references, distribution, and illustrations are presented here. The commonly occurring genera were Amphidinium, Coolia,Ostreopsis, Prorocentrum, and Thecadinium. Among the recorded species, 26 were found only in sand sediment,seven in macroalgal samples, and four were found in both sand and macroalgal samples. Of the 37 species, nine were potentially toxic. These results suggest that diversified benthic dinoflagellates including several potentially toxic species occur in sand sediment and macroalgae in the intertidal zone along the coasts of Jeju Island. The morphological features of the identified species were more or less similar to observations made by previous studies in Korea and elsewhere. The presence of known toxic species may indicate a potential risk of toxicity in the marine ecosystem of Jeju Island. The present study can be helpful for further detailed taxonomic, toxicological, molecular phylogenetic studies and may help in the management and conservation of Jeju Island’s marine ecosystem.
Aligizaki K, Nikolaidis G. 2006. The presence of the potentially toxic genera Ostreopsis and Coolia (Dinophyceae) in the North Aegean Sea, Greece. Harmful Algae 5: 717– 730.
Aligizaki K, Nikolaidis G. 2008. Morphological identification of two tropical dinoflagellates of the genera Gambierdiscus and Sinophysis in the Mediterranean Sea. J Biol Res–Thessalon 9: 75–82.
Aligizaki K, Nikolaidis G, Fraga S. 2008. Is Gambierdiscus expanding to new areas? Harmful Algae News 36: 6-7.
Al-Qassab S, Lee WJ, Murray S, Simpson AGB, Patterson DJ. 2002. Flagellates from stromatolites and surrounding sediments in Shark Bay, Western Australia. Acta Protozool 41: 91–144.
Al-Yamani FY, Saburova MA. 2010. Illustrated Guide on the Flagellates of Kuwait’s Intertidal Soft Sediments. Kuwait Institute for Scientific Research, Safat.
Andersen RA, Kawachi M. 2005. Traditional microalgae isolation techniques. In: Algal Culturing Techniques (Andersen RA, ed). Elsevier Academic Press, San Diego, CA, pp 83-101.
Bagnis R, Bennett J, Prieur C, Legrand AM. 1985. The dynamics of three toxic benthic dinoflagellates and the toxicity of ciguateric surgeonfish in French Polynesia. In: Toxic dinoflagellates (Anderson DM, White AW, Baden DG, eds). Elsevier, New York, pp 177–182.
Barbier M, Amzil Z, Mondeguer F, Bhaud Y, Soyer-Gobillard MO, Lassus P. 1999. Okadaic acid and PP2A cellular immunolocalization in Prorocentrum lima (Dinophyceae). Phycologia 38: 41-46.
Besada EG, Loeblich LA, Loeblich AR 3rd. 1982. Observations on tropical, benthic dinoflagellates from ciguatera-endemic areas: Coolia, Gambierdiscus, and Ostreopsis. Bull Mar Sci 32: 723-735.
Biecheler B. 1952. Recherches sur les Péridiniens. Bull Biol France et Belg, Suppl 36: 149.
Bomber JW, Norris DR, Mitchell LE. 1985. Benthic dinoflagellates associated with ciguatera from the Florida Keys. II. Temporal, spatial and substrate heterogeneity of Protocentrum lima. In: Toxic Dinoflagellates (Anderson DM, White AW, Baden DG, eds). Elsevier, New York, pp 45-50.
Carlson RD. 1984. Distribution, periodicity and culture of benthic/epiphytic dinoflagellates in a ciguatera endemic region of the Caribbean. PhD Dissertation. Southern Illinois University, Carbondale, Illinois, USA.
Carty S, Cox ER. 1986. Kansodinium gen. nov. and Durinskia gen. nov.: two genera of freshwater dinoflagellates (Pyrrhophyta). Phycologia 25: 197–204.
Chinain M, Faust MA, Pauillac S. 1999. Morphology and molecular analyses of three toxic species of Gambierdiscus (Dinophyceae): G. pacificus, sp. nov., G. australes, sp. nov., and G. polynesiensis, sp. nov. J Phycol 35: 1282- 1296.
Claparède E, Lachmann J. 1859. Études sur les Infusoires et les Rhizopodes 2. Mém Inst Nat Génev 6: 261–482.
Cortés-Altamirano R, Sierra-Beltrán AP. 2003. Morphology and taxonomy of Prorocentrum mexicanum and reinstatement of Prorocentrum rhathymum (Dinophyceae). J Phycol 39: 221–225.
Dodge JD. 1982. Marine Dinoflagellates of the British Isles. Her Majesty’s Stationery Office, London.
Dujardin F. 1841. Histoire naturelle des Zoophytes Infusoires, comprenant la physiologie et la classification de ces animaux et la maniere de les étudier à l’aide du microscope. Librairie encyclopédique de Roret, Paris.
Elbrächter M, Faust MA. 2002. IOC Taxonomic Reference List of Toxic Plankton Algae: Dinoflagellates (Class Dinoflagellata, Dinophyceae, Phylum Dinophyta) Order Prorcentrales, Intergovernmental Oceanographic Commission of UNESCO. http://ioc.unesco.org/hab/ data4taxlist. Accessed 03 April 2013, pp 23-29.
Faust MA. 1990. Morphologic details of six benthic species of Prorocentrum (Pyrrophyta) from a mangrove island, Twin Cays, Belize, including two new species. J Phycol 26: 548–558.
Faust MA. 1995. Observation of sand-dwelling toxic dinoflagellates (Dinophyceae) from widely differing sites, including two new species. J Phycol 31: 996-1003.
Faust MA. 1996. Dinoflagellates in a mangrove ecosystem, Twin Cays, Belize. Nova Hedwigia 112: 445-458.
Faust MA. 2009. Ciguatera-causing dinoflagellates in a coral- reef mangrove ecosystem, Belize. Atoll Res Bull 569: 1-32.
Faust MA, Gulledge RA. 2002. Identifying harmful marine dinoflagellates. Vol 42. Smithsonian Institution: Contributions from the United. States National Herbarium, Washington, DC.
Faust MA, Larsen J, Moestrup Ø. 1999. Potentially toxic phytoplankton. 3. Genus Prorocentrum (Dinophyceae). In: ICES Identification Leaflets for Plankton (Lindley JA, ed). International Council for the Exploration of the Sea, Copenhagen, Leaflet No. 184.
Faust MA, Morton SL, Quod JP. 1996. Further SEM study of marine dinoflagellates: the genus Ostreopsis (Dinophyceae). J Phycol 32: 1053–1065.
Fenchel T. 1988. Marine plankton food chain. Annu Rev Ecol Syst 18: 19-38.
Flø Jørgensen M, Murray S, Daugbjerg N. 2004a. A new genus of athecate interstitial dinoflagellates, Togula gen. nov., previously encompassed within Amphidinium sensu lato: Inferred from light and electron microscopy and phylogenetic analyses of partial large subunit ribosomal DNA sequences. Phycol Res 52: 284–299.
Flø Jørgensen M, Murray S, Daugbjerg N. 2004b. Amphidinium revisited. I. Redefinition of Amphidinium (Dinophyceae) based on cladistic and molecular phylogenetic analyses. J Phycol 40: 351–365.
Freudenthal HD, Lee JJ. 1963. Glenodinium halli n. sp. and Gyrodinium instriatum n. sp., dinoflagellates from New York Waters. J Protozool 10: 182-189.
Fritz L, Triemer RE. 1985. A rapid simple technique utilizing calcofluor white MR2 for the visualization of dinoflagellate thecal plates. J Phycol 21: 662–664.
Fukuyo Y. 1981. Taxonomical study on benthic dinoflagellates collected in coral reefs. Bull Jap Soc Sci Fish 47: 967–978.
Gilbert PM, Seitzinger S, Heil CA, Burkholder JM, Parrow MW, Codispoti LA, Kelly V. 2005. The role of eutrophication in the global proliferation of harmful algal blooms. Oceanography 18: 198–209.
Godhe A, Rehnstam-Holm AS, Karunasagar I, Karunasagar I. 2002. PCR detection of dinoflagellate cysts in field sediment samples from tropic and temperate environments. Harmful Algae 1: 361–373.
Hallegraeff G M. 1993. A review of harmful algal blooms and their apparent global increase. Phycologia 32: 79-99.
Han MS, Yoo KI. 1983a. A taxonomical study on the dinoflagellates in Jinhae bay, I: Armored and unarmored dinoflagellates. Bull KORDI 5: 37–47.
Han MS, Yoo KI. 1983b. A taxonomical study on the dinoflagellates in Jinhae bay, II: Peridiniales. Bull KORDI 5: 49–67.
Hansen G, Turquet J, Quod JP, Ten-Hage L, Lugomela C, Kyewalyanga M, Hurbungs M, Wawiye P, Ogongo B, Tunje S, Rakotoarinjanahary H. 2001. Potentially Harmful microalgae of the Western Indian Ocean: a Guide Based on a Preliminary Survey. I.O.C. Manuals and Guides 41. Intergovernmental Oceanographic Commission of UNESCO, Copenhagen.
Herdman EC. 1922. Notes on dinoflagellates and other organisms causing discolouration of the sand at Port Erin II. Proc Trans Liverpool Biol Soc 36: 15–30.
Holmes MJ. 1998. Gambierdiscus yasumotoi sp. nov. (Dinophyceae), a toxic benthic dinoflagellate from southeastern Asia. J Phycol 34: 661-668.
Holmes MJ, Lee FC, Teo SLM, Khoo HW. 1998. A survey of benthic dinoflagellates on Singapore Reefs. In: Harmful Algae (Reguera B, Blanco J, Fernandez ML, Wyatt T, eds.). Xunta de Galicia and Intergovernmental Oceanographic Commission of UNESCO, Santiago de Compostela, pp 50-51.
Hoppenrath M. 2000a. A new marine sand-dwelling Prorocentrum species, P. clipeus sp. nov. (Dinophyceae, Prorocentrales) from Helgoland, German Bight, North Sea. Europ J Protistol 36: 29-33.
Hoppenrath M. 2000b. Morphology and taxonomy of six marine sand-dwelling Amphidiniopsis species (Dinophyceae, Peridiniales), four of them new, from the German Bight, North Sea. Phycologia 39: 482–497.
Hoppenrath M, Elbrächter M. 1998. Roscoffia capitata (Dinophyceae) refound: notes on morphology and biology. Phycologia 37: 450-457.
Hoppenrath M, Leander BS. 2007. Morphology and phylogeny of the pseudocolonial dinoflagellates Polykrikos lebourae and Polykrikos herdmanae n. sp. Protist 158: 209-227.
Hoppenrath M, Schweikert M, Elbrächter M. 2003. Morphological reinvestigation and characterization of the marine, sand-dwelling dinoflagellate Adenoides eludens (Dinophyceae). Eur J Phycol 38: 385–394.
Hoppenrath M, Elbrächter M, Halliger H, Koeman RPT, Krakhmalnyy A, Surek B, Erler K, Luckas B. 2007a. First records of the benthic, bloom-forming, non-toxic dinoflagellate Thecadinium yashimaense (Dinophyceae) in Europe: with special emphasis on the invasion in the North Sea. Helgol Mar Res 61: 157-165.
Hoppenrath M, Horiguchi T, Miyoshi Y, Selina M, Taylor mFJR, Leander BS. 2007b. Taxonomy, phylogeny, biogeography, and ecology of Sabulodinium undulatum (Dinophyceae), including an emended description of the species. Phycol Res 55: 159-175.
Hoppenrath M, Selina M, Yamaguchi A, Leander B. 2012. Morphology and molecular phylogeny of Amphidiniopsis rotundata sp. nov. (Peridiniales, Dinophyceae), a benthic marine dinoflagellate. Phycologia 51: 157-167.
Horiguchi T. 1995. Amphidinella sedentaria gen. et sp. nov. (Dinophyceae), a new sand-dwelling dinoflagellate from Japan. Phycol Res 43: 93-99.
Horiguchi T, Tamura M, Katsumata K, Yamaguchi A. 2012. Testudodinium gen. nov. (Dinophyceae), a new genus of sand-dwelling dinoflagellates formerly classified in the genus Amphidinium. Phycol Res 60: 137-149.
Hu T, deFreitas ASW, Doyle J, Jackson D, Marr J, Nixon E, Pleasance S, Quilliam MA, Walter JA, Wright JLC. 1993. New DSP toxin derivatives isolated from toxic mussels and the dinoflagellates, Prorocentrum lima and Prorocentrum concavum. In: Toxic Phytoplankton Blooms in the Sea (Smayda TJ, Shimizu Y , eds). Elsevier, Amsterdam, pp 507-512.
Hulburt EM. 1957. The taxonomy of unarmored Dinophyceae of shallow embayments on Cape Cod, Massachusetts. Biol Bull 112: 196-219.
Iwataki M. 2008. Taxonomy and identification of the armored dinoflagellate genus Heterocapsa (Peridiniales, Dinophyceae). Plankton Benthos Research 3: 135-142.
Jeong HJ, Lim AS, Jang SH, Yih WH, Kang NS, Lee SY, Yoo YD, Kim HS. 2012a. First Report of the epiphytic dinoflagellate Gambierdiscus caribaeus in the temperate waters off Jeju Island, Korea: morphology and molecular characterization. J Eukaryot Microbiol 59: 637–650.
Jeong HJ, Yih W, Kang NS, Lee SY, Yoon EY, Yoo YD, Kim HS, Kim JH. 2012b. First report of the epiphytic benthic dinoflagellates Coolia canariensis and Coolia malayensis in the waters off Jeju Island, Korea: Morphology and rDNA Sequences. J Eukaryot Microbiol 59: 114–133.
Kang NS, Jeong HJ, Lee SY, Lim AS, Lee MJ, Kim HS, Yih WH. 2013. Morphology and molecular characterization of the epiphytic benthic dinoflagellate Ostreopsis cf. ovata in the temperate waters off Jeju Island, Korea. Harmful Algae 27: 98-112.
Kim HS, Yih WH, Kim JH, Myung G, Jeong HJ. 2011. Abundance of epiphytic dinoflagellates from coastal waters off Jeju Island, Korea during autumn 2009. Ocean Sci J 46: 205-209.
Kofoid CA, Swezy O. 1921. Memoir of the University of California Vol 5: The Free-Living Unarmored Dinoflagellata. University of California Press, Berkeley, CA.
Larsen J. 1985. Algal studies of the Danish Wadden Sea II. A taxonomic study of psammobious dinoflagellates. Opera Bot 79: 14–37.
Larsen J, Nguyen NL. 2004. Potentially toxic microalgae of Vietnamese waters. Opera Bot 140: 5–216.
Larsen J, Patterson DJ. 1990. Some flagellates (Protista) from tropical marine sediments. J Nat Hist 24: 801–937.
Leaw CP, Lim PT, Cheng KW, Ng BK, Usup G. 2010. Morphology and molecular characterization of a new species of thecate benthic dinoflagellate, Coolia malayensis sp. nov. (Dinophyceae). J Phycol 46: 162–171
Lebour MV. 1925. The Dinoflagellates of Northern Seas. Marine Biological Association, Plymouth.
Lee KH, Jeong HJ, Park K, Kang NS, Yoo DY, Lee MJ, Lee JW, Lee S, Kim T, Kim HS, Noh JH. 2013. Morphology and molecular characterization of the epiphytic dinoflagellate Amphidinium massartii, isolated from the temperate waters off Jeju Island, Korea. Algae 28: 213-231.
Lee SG, Park JS, Kim HG. 1993. Taxonomy of marine toxic flagellates occurring in the southern coastal waters of Korea. Bull Nat Fish Res Dev Agency 48: 1-23.
Lehane L, Lewis RJ. 2000. Ciguatera: recent advances but the risk remains. Int J Food Microbiol 61: 91–125.
Lim AS, Jeong HJ, Jang TY, Kang NS, Lee SY, Yoo YD, Kim HS. 2013. Morphology and molecular characterization of the epiphytic dinoflagellate Prorocentrum cf. rhathymum in temperate waters off Jeju Island, Korea. Ocean Sci J 48: 1–17.
Loeblich III AR. 1965. Dinoflagellate nomenclature. Taxon 14: 15–18.
Loeblich III AR, Sherley JL, Schmidt RJ. 1979. The correct position of flagellar insertion in Prorocentrum and description of Prorocentrum rhathymum sp. nov. (Pyrrhophyta). J Plankton Res 1: 113–120.
Maranda L, Shimizu Y. 1996. Amphidinium operculatum var. nov. gibbosum (Dinophyceae), a free-swimming marine species producing cytotoxic metabolites. J Phycol 32: 873-879.
Marr JC, Jackson AE, McLachlan JL. 1992. Occurrence of Prorocentrum lima, a DSP toxin-producing species from the Atlantic coast Canada. J Appl Phycol 4: 17-24.
Mohammad-Noor N, Daugbjerg N, Moestrup O, Anton A. 2007. Marine epibenthic dinoflagellates from Malaysia: A study of live cultures and preserved samples based on light and scanning electron microscopy. Nordic J Botany 24: 629–690.
Morton SL, Faust MA. 1997. Survey of toxic epiphytic dinoflagellates from the Belizean Barrier Reef Ecosystem. Bull Mar Sci 61: 899–906.
Murray S. 2003. Diversity and phylogenetics of sand-dwelling dinoflagellates from Southern Australia. PhD Dessertation. University of Sydney, Sydney, Australia.
Murray S, Flø Jørgensen M, Daugbjerg N, Rhodes L. 2004. Amphidinium revisited. II. Resolving species boundaries in the Amphidinium operculatum species complex (Dinophyceae), including the descriptions of Amphidinium trulla sp. nov. and Amphidinium gibbosum comb. nov. J Phycol 40: 366–382.
Murray S, Nagahama Y, Fukuyo Y. 2007. Phylogenetic study of benthic, spine-bearing prorocentroids, including Prorocentrum fukuyoi sp. nov. Phycol Res 55: 91–102.
Murray S, Patterson DJ. 2002. The benthic dinoflagellate genus Amphidinium in south-eastern Australian waters, including three new species. Eur J Phycol 37: 279–298.
Murray S, Patterson DJ. 2004. Cabra matta gen. nov., sp. nov., a new benthic, heterotrophic dinoflagellate. Eur J Phycol 39: 229-234
Nakajima I, Oshima Y, Yasumoto T. 1981. Toxicity of benthic dinoflagellates in Okinawa. Bull Jpn Soc Sci Fish 47: 1029–1033.
Nakajima I, Oshima Y, Yasumoto T. 1981. Toxicity of benthic dinoflagellates in Okinawa. Bull Jpn Soc Sci Fish 47: 1029-1033.
Okolodkov YB, Campos-Bautista G, Gárate-Lizárraga I, González-González JAG, Hoppenrath M, Arenas V. 2007. Seasonal changes of benthic and epiphytic dinoflagellates in the Veracruz reef zone, Gulf of Mexico. Aquat Microb Ecol 47: 223–237.
Pang IC, Rho HK, Lee JH, Lie HJ. 1996. Water mass distribution and seasonal circulation northwest of Cheju Island in 1994. J Korean Fish Soc 29: 862–875.
Parsons ML, Preskitt LB. 2007. A survey of epiphytic dinoflagellates from the coastal waters of the island of Hawaii. Harmful Algae 6: 658–669.
Pearce I, Marshall JA, Hallegraeff GM. 2001. Toxic epiphytic dinoflagellates from east coast Tasmania, Australia. In: Harmful algal blooms 2000 (Hallegraeff GM, Blackburn SI, Bolch C J, Lewis RJ, eds). Intergovernmental Oceanographic Commission of UNESCO, Tasmania, Australia, pp 54-57.
Penna A, Vila M, Fraga S, Giacobbe MG, Andreoni F, Riobó P, Vernezi C. 2005. Characterization of Ostreopsis and Coolia (Dinophyceae) isolates in the western mediterranean sea based on morphology, toxicity and internal transcribed spacer 5.8S rDNA sequences. J Phycol 41: 212–225.
Pienaar RN, Sakai H, Horiguchi T. 2007. Description of a new dinoflagellate with a diatom endosymbiont, Durinskia capensis sp. nov. (Peridiniales, Dinophyceae) from South Africa. J Plant Res 120: 247–258.
Pistocchi R, Pezzolesi L, Guerrini F, Vanucci S, Dell’Aversano C, Fattorusso E. 2011. A review on the effects of environmental conditions on growth and toxin production of Ostreopsis ovata. Toxicon 57: 421-428.
Prokic I, Brümmer F, Brigge T, Görtz HD, Gerdts G, Schütt C, Ellbrächter M, Müler WE. 1998. Bacteria of the genus Roseobacter associated with the toxic dinoflagellate Prorocentrum lima. Protist 149: 347-357.
Rhodes L. 2011. World-wide occurrence of the toxic dinoflagellate genus Ostreopsis Schmidt. Toxicon 57: 400-407.
Saburova M, Al-Yamani F, Polikarpov I. 2009. Biodiversity of free-living flagellates in Kuwait’s intertidal sediments. BioRisk 3: 97-110.
Schiller J. 1933. Dinoflagellatae (Peridineae). Kryptogamen- Flora. Akad Verl Leipzig 10: 276–322.
Sechet V, Quillam MA, Rocher G. 1998. Diarrhetic shellfish poisoning (DSP) toxins in Prorocentrum lima in axenic and non-axenic batch culture: detection: detection of new compounds and kinetics of production. In: Harmful Algae (Reguera B, Blanco J, Fernandez ML, Wyatt T, eds). Xunta de Galicia and Intergovernmental Oceanographic Commission of UNESCO, Santiago de Compostela, pp 485-488.
Selina M, Hoppenrath M. 2008. An emended description of Amphidiniopsis arenaria Hoppenrath 2000, based on material from the Sea of Japan. Eur J Protistol 44: 71-79.
Selina MS, Levchenko EV. 2011. Species composition and morphology of dinoflagellates (Dinophyta) of epiphytic assemblages of Peter the Great Bay in the Sea of Japan. Rus J Mar Biol 37: 23-32.
Shim JH. 1994. Illustrated Encyclopedia of Fauna and Flora of Korea, Vol 34. Marine Phytoplankton. Ministry of Education, Seoul. (in Korean)
Shim JH, Shin EY, Choi JK. 1981. A taxonomical study on the dinoflagellates of the coastal waters in the vicinity of Yeosu, Korea. J Oceanol Soc Korea 16: 57- 98. (in Korean)
Steidinger KA, Tangen K. 1997. Dinoflagellates. In: Identifying Marine Phytoplankton (Tomas CR, ed). Academic Press, San Diego, CA, pp 387–570.
Tamura M, Iwataki M, Horiguchi T. 2005. Heterocapsa psammophila sp. nov. (Peridiniales, Dinophyceae), a new sand-dwelling marine dinoflagellate. Phycol Res 53: 303–311.
Tindall DR, Miller DM, Bomber JW. 1989. Culture and toxicity of dinoflagellates from ciguatera endemic regions of the world. Toxicon 27: 83.
Turquet J. 1997. Etude écologique et toxicologique des Dinophycées benthiques inféodées aux intoxications alimentaires dans le Sud-ouest de l’Océan Indien. PhD Dissertation, Paris VII University, Paris, France.
Turquet J, Quad JP, Couté A, Faust MA. 1998. Assemblage of benthic dinoflagellates and monitoring of harmful species in Reunion Island, SW Indian Ocean, 1993–1996. In: Harmful algae (Reguera B, Blanco J, Fernández ML, Wyatt T, eds). Xunta de Galicia and Intergovernmental Oceanographic Commission of UNESCO, Santiago de Compostela, pp 44–47.
Yasumoto T, Seino N, Murakami Y, Murata M. 1987. Toxins produced by benthic dinoflagellates. Biol Bull 172: 128–131.
Yoo KI, Lee JB. 1986. Taxonomical studies on dinoflagellates in Masan Bay 1. Genus Prorocentrum Ehrenberg. J Kor Soc Oceanogra 21: 46-55.
Yoshimatsu, S, Toriumi S, Dodge JD. 2004. Morphology and taxonomy of five marine sand-dwelling Thecadinium species (Dinophyceae) from Japan, including four new species: Thecadinium arenarium sp. nov., Thecadinium ovatum sp. nov., Thecadinium striatum sp. nov. and Thecadinium yashimaense sp. nov. Phycol Res 52: 211–223.