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ISSN : 2287-8327

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Vol.37 No.1
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Effect of N-acetyl-L-cysteine on human chronic myeloid leukemia cells KCL22 treated with mitomycin C

Journal of Ecology and Environment / Journal of Ecology and Environment, (P)2287-8327; (E)2288-1220
2014, v.37 no.1, pp.31-34
https://doi.org/10.5141/ecoenv.2014.004
Anna Simonyan (Yerevan State University)

Galina Hovhannisyan (Yerevan State University)
Rouben Aroutiounian (Yerevan State University)
Anna, S. , Galina, H. , & Rouben, A. (2014). Effect of N-acetyl-L-cysteine on human chronic myeloid leukemia cells KCL22 treated with mitomycin C. Journal of Ecology and Environment, 37(1), 31-34, https://doi.org/10.5141/ecoenv.2014.004
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Abstract

The effectiveness of N-acetyl-L-cysteine (NAC) to protect blood cells against Mitomycin C (MMC) induced genotoxicitywas investigated in human chronic myeloid leukemia cells (KCL22) using the alkaline comet assay. The comet assay wasselected as sensitive and rapid method for analysis of DNA damage and repair in individual cells. NAC treatment alonedid not produce any damage in KCL22 cell. But NAC was found to be effective in reducing genotoxic damage in KCL22cells exposed to MMC. These results confirm the literature data that, given the safety and ability to reduce DNA damage. NAC may be useful to prevent drug-mediated genotoxicity.

keywords
comet assay, genotoxicity prevention, mitomycin C, N-acetyl-L-cysteine

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