ISSN : 2287-8327
Molecular identification of fruit bats, natural host of Nipah virus in Bangladesh, based on DNA barcode
Walid Hassan (Department of Biotechnology and Genetic Engineering, Faculty of Biological Science, Islamic University, Kushtia 7100, Bangladesh)
Afia Afsin (Department of Biotechnology and Genetic Engineering, Faculty of Biological Science, Islamic University, Kushtia 7100, Bangladesh)
Md. Mahfuzur Rahman (International Centre for Diarrhoeal Disease Research, Bangladesh (icddr,b), Dhaka 1212, Bangladesh)
Md. Tanvir Rahman (Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh)
임상진 (강원대학교)
Oh Yeonsu (College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University, Chuncheon 24341, Korea)
박영철 (강원대학교)
Hossain Md. Faruquee (Department of Biotechnology and Genetic Engineering, Faculty of Biological Science, Islamic University, Kushtia 7100, Bangladesh)
Md. Mafizur Rahman (Department of Biotechnology and Genetic Engineering, Faculty of Biological Science, Islamic University, Kushtia 7100, Bangladesh)
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Abstract
Background: Fruit bats are natural carriers of Nipah virus (NiV ). The primary objective of this study is to identify potential reservoir species in a selected geographic regions. It is necessary to determine an accurate species identification of the associated reservoir bat species distributed in a specific region. Results: In this study, we collected 20 different bat specimens from the NiV-prone area of the Kushtia district. Among these, 14 were tissue samples (BT-1–14) and six were fecal sam- ples (BF-1–6). We used the mitochondrial gene cytochrome b, one of the most abundant and frequently used genetic markers, for polymerase chain reaction amplification and se- quencing. Out of the 20 samples, 12 tissue samples and 2 fecal samples were successfully amplified and sequenced. However, two tissue samples and four fecal samples yielded chimeric sequences, rendering them unsuitable for annotation. The sequences of the suc- cessfully amplified samples were compared to those deposited in the National Center for Biotechnology Information database using basic local alignment search tool to identify the bat specimen collected. The study identified six different bat species using both morpho- logical and genetic data, which may carriers of the NiV. Conclusions: Our results suggest that additional research should be conducted to gath- er more information on fruit bats from different localities across the country. The study contributes to the establishment of appropriate measures for NiV carrying disease control and management.
- keywords
- cytochrome b, ecology, Nipah virus, Pteropus, zoonotic
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