Traditional deception detection methods had examined the difference of one’s autonomic physiological responses through asking crime-related and crime-unrelated questions. There has been a continuing controversy regarding the accuracy and validity of the test, and thus, many researchers were motivated to explore and develop alternative efficient methods of detection in which one of them is known as P300-based Complex Trial Protocol (CTP). The P300-based CTP detects deception through comparing the P300 amplitudes between probe and irrelevant stimuli and is known as a counterstrategy of countermeasures. However, many previous studies have used countermeasures created from Rosenfeld et al.’s work (2008).The present study initially conducted a survey asking open-ended questions about the countermeasure use to acquire participant-oriented countermeasures for the main experiment. Then, the study aimed to evaluate whether the CTP can accurately detect deception even in the use of survey-based countermeasures. We firstly selected a set of participant-oriented countermeasures through survey questions. Then, a total of 50 participants were divided into three groups (innocent, guilty, and countermeasures) and performed the CTP. Those assigned to the countermeasures group covertly performed mental countermeasures during the CTP. The results of P300 amplitude analysis revealed that the guilty group’s P300 amplitude of probe stimuli was significantly larger than that of irrelevant stimuli. Countermeasures group also had a significantly larger P300 amplitude for probe stimuli compared to irrelevant stimuli, even in the use of countermeasures. The results of bootstrapped amplitude difference (BAD) showed a detection accuracy rate of 81.25%, 82.35%, 82.35% for the innocent, guilty, and countermeasures groups, respectively. These findings demonstrate that the CTP can obtain a high detection rate in participant-oriented countermeasures and suggest the potential use of the CTP in the
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