ISSN : 1225-3480
피조개, Scapharca broughtonii (Schrenck) 정자의 냉장보존을 위해 희석액에 따른 보존효과를 비교하였다. 피조개 정액을 채취하여 600 mM NaCl, Stein solution, 300 mM,600 mM glucose (10 mM Hepes-pH 7.8), Ringer’s solution (230 mM NaCl, 8 mM KCl, 2 mM CaCl2, 3.7mM MgCl2, 0.2 mM NaHCO3, 10 mM Hepes-pH 7.8),20%, 25% ASW (NaCl 2.7 g + KCl 0.07 g + CaCl20.12 g + MgCl2 0.46 g + NaHCO3 0.05 g + distilled water 100 ml) 를 각각 사용하여 4℃에서 보관하였다. 피조개 정자의 보존효과는 정자의 운동성과 생존율로 평가였다. 피조개 정자는 Ringer's solution에서 7일간 냉장보전하였을 때운동성과 생존율을 가장 높았으며, 희석액의 적정 pH는 7.5이었다.
The effects of diluents composition on cold storage for Scapharca broughtonii (Schrenck) sperm were examined in the percentage of sperm activity and survival rate. Various diluents of glucose solutions (10 mM Hepes-pH 7.8), 600 mM NaCl, stein solution, Ringer’s solution(230mM NaCl, 8 mM KCl, 2 mM CaCl2, 3.7 mM MgCl2, 0.2 mM NaHCO3, 10 mM Hepes-pH 7.8), 20%, 25% ASW (NaCl 2.7 g + KCl 0.07 g + CaCl2 0.12 g + MgCl2 0.46 g + NaHCO3 0.05 g + distilled water 100 ml) were used to store th sperm at 4℃. The storage effect was evaluated using sperm activity and survival rate. Ringer's solution was found to be better diluents which maintained high activity and survival rate of sperm for a storage period of 7 days. Optimal pH of diluents to store the sperm at 4℃ is 7.5.
Alavi S.M.H. and J. Cosson (2005) Sperm motility in fishes. I. Effects of temperature and pH: a review, Cell Biol. Int. 29: 101-110.
Ansah G.A., D.C. Crober, R.B Buckland, A.E. Sefton and B.W. Kennedy (1980) Artificial insemination of individually caged broiler breeders. 1. Reproductive performance of males relation to age and strain of females. Poultry Sci., 59: 428-437.
Billard, R., J. Cosson, S.B. Noveiri and M. Pourkazemi (2004) Cryopreservation and short-term storage of sturgeon sperm, a riview. Aquaculture, 236: 1-9.
Blaxter J.H.S (1953) Sperm storage and cross fertilization of spring and autumn spawning herring. Nature, 172: 1189-1190.
Blom, E. (1950) A one-minute live-dead sperm stain by means ofeosin-nigrosin. Fertil. Steril., 1: 176-177.
Butts A.E., E.A. Trippel and M.K. Litvak (2009) The effect of sperm to egg ratio and gamete contact time on fertilization success in Atlantic cod Gadus morhua L. Aquaculture, 286: 89–94.
Carlos Frederico Ceccon Lanes, Marcelo Okamoto, Paulo Varoni Cavalcanti, Tiago Collares, Vinicius Farias Campos, Joao Carlos Deschamps, Ricardo Berteaux Robaldo, Luis Fernando Marins and Luis Andre Sampaio (2008) Cryopreservation of Brazilian flounder (Paralichthys orbignyanus) sperm. Aquaculture, 275(1-4): 361-365.
Chang Y.J., Y.J. Chang, H.K. Lim, J.K. Lee and Y.J. Park (2002) Coldstorage of milt from four species of flatfish. J. Fish. Sci. Technol., 5: 64-74.
Chao, N. H., H. P. Chen and I. C. Liao (1975) Study on cryogenic preservation of grey mullet sperm. Aquaculture, 5: 389-406.
Chun Y.Y., Na G.H. and Choi E.J. (1991) Mass morta1ity of arkshell, Anadara broughtonii SCHRENCK seed1ing with marine ecologica1 characteristics. Bull. Kor. Fish. Soc., 24: 70-78.
DeGraaf, J. D. and D. L. Berlinsky (2004) Cryogenic and refrigerated storage of Atlantic cod (Gadus morhua) and haddock (Melanogrammus aeglefinus) spermatozoa. Aquaculture, 234: 527-540.
Ding Shuyan , Jiachun Ge, Chen Hao, Mingsheng Zhang, Weihui Yan, Zhiqiang Xu, Jianlin Pan, Songlin Chen, Yongsheng Tian and Yahong Huang (2009) Long-term cryopreservation of sperm from Mandarin fish Siniperca chuatsi. Animal Reproduction Science, 113(1-4): 229-235.
Donoghue A.M. and G.J. Wishart (2000) Storage of poultry semen. Anim. Prprod. Sci., 62: 213-232.
Fribourgh, J.H. 1966. The app1ication of a differentia1 staining method to 10w-temperature studies on goldfish spermatozoa. Prog. Fish-Cu1t., 28: 227-231.
Friedler S, Raziel A, Strassburger D, Schachter M, Soffer Y, Ron-EI R. (2002) Factors influencing the outcome of ICSI in patients with obstructive and non-obstructive azoospermia: a comparative study. Hum Reprod, 17: 3114-3121.
Hara, S., J. T. Canto and J. M. E. Almendras (1982) A comparative study of various extenders for milkfish, Chanos chanos (Forsskal), sperm preservation. Aquaculture, 28: 339-346.
Hauser R., Yogev L., Amit A., Yavetz H., Botchan A., Azem F., Lessing J.B. and Ben-Yosef D. (2005) Severe hypospermatogenesis in cases of nonobstructive azoospermia: Should we use or frozen testicular spermatozoa. J. andrology, 26:772-8.
He S. and L.C. Woods III (2003) Effects of glycine and alanine on short-term storage and cryopreservation of striped bass (Morone saxatilis) spermatozoa. Cryobiology, 46: 17–25.
Jinga Rongyan, Changjiang Huanga, Chenglian Baia, Robert Tanguaya and Qiaoxiang Donga (2009) Optimization of activation, collection, dilution, and storage methods for zebrafish sperm. Aquaculture, 290(1-2): 165-171.
Kho K.H., Kang K.H. and Kim J.M. (2003) Effect of diluents on the cold storage of sperm in sea urchin, Hemicentrotus pulcherrimus. Dev. Reprod., 7(1):9-13.
Kho K.H. (2004) Physiologycal approach to improve solutions for liquid storage of semen and artificial fertilization in the brown trout (Salmo trutta). Korean J. Ichthyol., 16(3): 215-222.
Kim B.H., Min K.S., Lee S.J., Park K.Y., An C.M., and Min B.H. (2006) Effect of Temperature on Induced Sexual Maturation of the arkshell, Scapharca broughtonii (Schrenck) Broodstock. The Korean Journal of Malacology, 22(2): 175-182.
Kim J.B., Lee S.Y., Jung C.G., Jung C.S. and So S.G. (2007) The effects of the spat planting time and environmental factors in the arkshell, Scapharca broughtonii Schrenck culture. J. Aquaculture in Korea, 20(1): 31-40.
Lim, H. K., C. M. An, M. H. Son, M. W. Park and Y. J. Park (2005) Effect of diluents for cold storage of olive flounder (Paralichthys olivaceus) sperm. J. Kor. Fish. Soc., 38: 232-238.
McNiven, M.A., R.K. Gallant and G.F. Richardson (1993) Fresh storage of rainbow trout (Oncorhynchus mykiss) semen using a non-aqueous medium. Aquaculture, 109: 71-82.
Muchlisin Z.A. , R. Hashim and A.S.C. Chong (2004) Preliminary study on the cryopreservation of tropical bagrid catfish (Mystus nemurus) spermatozoa; the effect of extender and cryoprotectant on the motility after short-term storage, Theriogenology, 62: 25–34.
Matteo O.Di , A.L. Langellotti, P. Masullo and G. Sansone (2009) Cryopreservation of the Mediterranean mussel (Mytilus galloprovincialis) spermatozoa. Cryobiology, 58(2): 145-150.
Ohta H. and T. Izawa (1996) Diluent for cool storage of Japanese eel (Anguilla japonica) spermatozoa. Aquaculure, 142: 107-118.
Park M.S., Lim H.J. and Kim P.J. (1998) Effect of environmental factors on the growth, glycogen and hemoglobin content of cultured Arkshell, Scapharca broughtonii. J. Kor. Fish. Soc., 31, 176-175.
Park M.S., Kang C. and Lee P. (2001) Reproductive cycle and biochemical composition of the ark shell Scaphaw broughtonii (Schrenck) in a southem coast bay of Korea. J. Shellfish Research, 20: 51-59.
S.L. Adams, J.F. Smith, R.D. Roberts, A.R. Janke, H.F. Kaspar, H.R. Tervit, P.A. Pugh, S.C. Webb and N.G. King (2004) Cryopreservation of sperm of the Pacific oyster (Carassostrea gigas): development of a practical method for commercial spat production, Aquaculture 242: 271-282.
Takayuki Kawamoto, Teruyoshi Narita, Kiyoshi Isowa, Hideo Aoki, Masahiro Hayashi, Akira Komaru and Hiromi Ohta (2007) Effects of cryopreservation methods on post-thaw motility of spermatozoa from the Japanese pearl oyster, Pinctada fucata martensii. Cryobiology, 54(1): 19-26.
Usuki H., M. Hamaguchi and H. Ishioka (1997) Long-term cryopreservation of Pacific oyster, Crassostrea gigas, sperm. Bull. Nansei Natl. Fish. Res. Inst. 30: 115–.123 (in Japanese with English abstracts).
Verapong Vuthiphandchai, Itthinan Thadsri and Subuntith Nimrat (2009) Chilled storage of walking catfish (Clarias macrocephalus) semen. Aquaculture, 296(1-2): 58-64.
Yao Z., L.W. Crim, G.F. Richardson and C.J. Emerson (2000) Motility, fertility and ultrastructural changes of ocean pout (Macrozoarces americanus L.) sperm after cryopreservation. Aquaculture, 181(3-4):361-375.
Zilli L., R. Schiavone, V. Zonno, C. Storelli and S. Vilella (2003) Evaluation of DNA damage in Dicentrarchus labrax sperm following cryopreservation. Cryobiology, 47(3): 227-235.
