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ISSN : 1225-3480
원유의 WSF가 참전복의 유생 및 치패 발생에 미치는 영향을 알아보기 위하여 발생소요시간, 발생률 및 부착률, 생존율,유생 및 부착치패의 각성장 그리고 수온에 따른 치패의 생존율및 아가미의 조직학적 변화를 조사하였다. 발생소요시간은 대조구에 비해 노출구에서 느리게 나타났다. 발생률 및 부착률은0.4 mg/L 이상의 농도에서 대조구에 비해 유의적인 감소를보였다 (P < 0.05). 참전복 유생의 생존율은 0.4 mg/L 이상에서, 부착치패는 2.4 mg/L 이상에서 유의적인 감소가 나타났다 (P < 0.05). 각성장은 2.4 mg/L 이상의 농도에서 대조구에 비해 감소하였다 (P < 0.05). 수온에 따른 치패의 생존율은 17℃와 25℃ 모두 노출농도에서 감소하였으며, 특히 고수온에서 전체적인 생존율이 낮게 나타났다. 또한 아가미에서나타난 조직학적 변화는 주로 새엽 상피세포 핵의 위축 및 상피세포의 공포화 그리고 상피층의 괴사를 동반한 붕괴 등이 관찰되었으며, 이는 17℃에 노출된 개체들 보다는 고수온인 25℃에 노출된 개체들에서 심하게 나타났다.
This study was carried out to investigate the toxic effect of WSF (Water Soluble Fraction) on development time, development rate, attachment rate, survival rate and growth of the larvae during the early life stage of the abalone, Haliotis discus hannai. Also, observed effect of water temperature on the survival rate and histological change of gill in the early young shell. In the abalone, it takes around 12 hours in all experimental groups. Development and attachment rate of the abalone significantly lower more than 0.4 mg/L WSF compared to control group (P < 0.05). Survival rate of abalone larva and spot was significantly lower more than 0.4 mg/L and 2.4 mg/L WSF compared to control group, respectively (P < 0.05). Shell growth of the abalone were significantly lower more than 2.4 mg/L WSF compared control group (P < 0.05). Survival rate lower more than 25℃ exposure group compared water temperature 17℃ exposure group in the early young shell. The gill of abalone exposed water temperature 17℃ and 25℃ was showed atrophy of nucleus and breakdown of the filament, vacuolation of filament epithelial cell.
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