ISSN : 1225-3480
피조개, Scapharca broughtonii (Schrenck) 정자의 동결보존 위해 동결높이에 따른 예비동결 (0, 2, 4, 6 ㎝), 희석제 (150 mM NaCl, 300 mM glucose, Ringer’s solution)와 동해방지제(glycerol, dimethylsulfoxide (DMSO),dimethylacetamide (DMA), methaol) 에 따른 효과를 정자의 운동성과 생존율로 비교하였다. 피조개 정자 동결보존에있어 예비동결은 액체질소표면으로부터 2 ㎝가 가장 효과적이었으며, 희석제로는 Ringer's solution이 동해방지제로는DMSO 10% 농도가 가장 적합하였다
This study was conducted to investigate protocol standardization for spermatozoa cryopreservation of the Scapharca broughtonii (Schrenck). Among the freezing rates, freezing at a height of 2 ㎝ above liquid nitrogen surface for 5 minute gave higher activity and survival rate. Among the various diluents, Ringer's solution was the best for S. broughtonii sperm cryopreservation. The suitability of cryoprotectants dimethylsulfoxide (DMSO), dimethylacetamide (DMA), glycerol and methanol were tested against three freezing rates. DMSO gave significantly higher activity and survival rates than others.
Adams S.L., Smith J.F., Roberts R.D., Janke A.R., Kaspar H.F., Tervit H.R., Pugh P.A., Webb S.C. and King N.G. (2004) Cryopreservation of sperm of the Pacific oyster (Crassostrea gigas): development of a practical method for commercial spat production. Aquaculture, 242: 271-282.
Anchordoguy T., Crowe J.H., Griffin F.J., Clark W.H. Jr. (1988) Cryopreservation of sperm from the marine shrimp Sicyonia ingentis. Cryobiology, 25(3): 238-43.
Billard, R., J. Cosson, S.B. Noveiri and M. Pourkazemi (2004) Cryopreservation and short-term storage of sturgeon sperm, a riview. Aquaculture, 236: 1-9.
Billard R. and Zhang T. (2001) Techniques of genetic resource banking in fish. In: P.F. Walson and W.V. Holt, Editors, Cryobanking the Genetic Resource:Wildlife Conservation for the Future. pp. 143–170, Taylor & Francis, New York
Blom E. (1950) A one-minute live-dead sperm stain by means ofeosin-nigrosin. Fertility and sterility, 1:176-177.
Bolla S., Holmefjord I. and Refstie T. (1987) Cryogenic preservation of Atlantic halibut sperm. Aquaculture. 65: 371-374.
Butts A.E., E.A. Trippel and M.K. Litvak (2009) The effect of sperm to egg ratio and gamete contact time on fertilization success in Atlantic cod Gadus morhua L. Aquaculture, 286: 89–94.
Chang Y.J., Y.J. Chang and H.K. Lim (1997) Cryopreservation of Tiger puffer (Takifugu rubripes) sperm. Development and Reproduction, 1(1): 29-36. [in Korean]
Chang Y.J., Y.J. Chang, H.K. Lim, J.K. Lee and Y.J. Park (2002) Coldstorage of milt from four species of flatfish. Journal of Fisheries Science and Technology, 5: 64-74. [in Korean]
Chao N.H., H.P. Chen and I.C. Liao (1975) Study on cryogenic preservation of grey mullet sperm. Aquaculture, 5: 389-406.
Chun Y.Y., Na G.H. and Choi E.J. (1991) Mass morta1ity of arkshell, Anadara broughtonii SCHRENCK seed1ing with marine ecological characteristics. Bulletin Korea Fisheris Society, 24:70-78. [in Korean]
Christiansen I.J. (1984) Part 1. Andrology of the normal male. In Reprodudtion in the Dog and Cat. pp 80-109. Bailliere Tindal, London.
DeGraaf J.D. and D.L. Berlinsky (2004) Cryogenic and refrigerated storage of Atlantic cod (Gadus morhua) and haddock (Melanogrammus aeglefinus) spermatozoa. Aquaculture, 234: 527-540.
Di Matteo O., Langellotti A.L., Masullo P., Sansone G. (2009) Cryopreservation of the Mediterranean mussel (Mytilus galloprovincialis) spermatozoa. Cryobiology, 58(2): 145-150.
Ding S., Ge J., Hao C., Zhang M., Yan W., Xu Z., Pan J., Chen S., Tian Y. and Huang Y. (2009) Long-term cryopreservation of sperm from Mandarin fish Siniperca chuatsi. Animal Reproduction Science, 113(1-4): 229-235.
Dong Q., Huang C., Eudeline B. and Tiersch T.R. (2005) Tierscha. Systematic factor optimization for cryopreservation of shipped sperm samples of diploid Pacific Oysters, Crassostrea gigas. Cryobiology, 51(2): 176-97.
Fribourgh, J.H. (1966) The app1ication of a differentia1 staining method to 10w-temperature studies on goldfish spermatozoa. Progressive Fish-Culturist, 28:227-231.
Hara S., J.T. Canto and J.M.E. Almendras (1982) A comparative study of various extenders for milkfish, Chanos chanos (Forsskal), sperm preservation. Aquaculture, 28: 339-346.
Harvey B. (1983) Cryopreservation of Sarotherodon mossambicus spermatozoa. Aquaculture, 32: 313-320.
He S. and L.C. Woods III (2003) Effects of glycine and alanine on short-term storage and cryopreservation of striped bass (Morone saxatilis) spermatozoa. Cryobiology, 46: 17–25.
Kawamoto T., Narita T., Isowa K., Aoki H., Hayashi M., Komaru A., Ohta H. (2007) Effects of cryopreservation methods on post-thaw motility of spermatozoa from the Japanese pearl oyster, Pinctada fucata martensii. Cryobiology, 54(1): 19-26.
Kho K.H., Kang K.H. and Kim J.M. (2003) Effect of diluents on the cold storage of sperm in sea urchin, Hemicentrotus pulcherrimus. Development and Reproduction, 7(1): 9-13. [in Korean]
Kho K.H. (2007) Milt property and sperm motility of panther puffer, Takifugu pardalis. Korean Journal of Ichthyology, 19(2): 168-172. [in Korean]
Kim B.H., Min K.S., Lee S.J., Park K.Y., An C.M., and Min B.H. (2006) Effect of Temperature on Induced Sexual Maturation of the arkshell, Scapharca broughtonii (Schrenck) broodstock. The Korean Journal of Malacology, 22(2): 175-182. [in Korean]
Kim J.B., Lee S.Y., Jung C.G., Jung C.S. and So S.G. (2007) The effects of the spat planting time and environmental factors in the arkshell, Scapharca broughtonii Schrenck culture. Journal of Aquaculture, 20(1): 31-40. [in Korean]
Kuwano K. and N. Saga (2000) Cryopreservation of marine algae: Cryopreservation of marine algae:Applications in biotechnology. In: Figerman, M. and R. Nagabhusshanam. ed. Recent advances in marine biotechnology. Volume 4: Aquaculture. Part A, Seaweeds and invertebrates. pp. 23-40. Science Pubulishers Inc., New Hampshire.
Lanes C.F.C., Okamoto M., Cavalcanti P.V., Collares T., Campos V.F., Deschamps J.C., Robaldo R.B., Marins L.F. and Sampaio L.A. (2008) Cryopreservation of Brazilian fl ounder (Paralichthys orbignyanus) sperm. Aquaculture. 275: 361-65
Lim H.K., Kho K.H. and Chang Y.J. (1997) Effect of diluents on the short-term storage of sperm in Black seabream, Acanthopagrus schlegeli. Journal of the Korean Fisheries Society, 30(2): 211-215.
Lim H.K., C.M. An, M.H. Son, M.W. Park and Y.J. Park (2005) Effect of diluents for cold storage of olive flounder (Paralichthys olivaceus) sperm. Journal of the Korean Fisheries Society, 38: 232-238.
Mazur P. and Cole K.W. (1985) Influence of cell concentration on the contribution of unfrozen fraction and salt concentration to the survival of slowly frozen human erythrocytes. Cryobiology, 22:509-536.
McNiven M.A., R.K. Gallant and G.F. Richardson (1993) Fresh storage of rainbow trout (Oncorhynchus mykiss) semen using a non-aqueous medium. Aquaculture, 109: 71-82.
Muchlisin Z.A., R. Hashim and A.S.C. Chong (2004) Preliminary study on the cryopreservation of tropical bagrid catfish (Mystus nemurus) spermatozoa; the effect of extender and cryoprotectant on the motility after short-term storage, Theriogenology, 62: 25-34.
Ohta H. and T. Izawa (1996) Diluent for cool storage of Japanese eel (Anguilla japonica) spermatozoa. Aquaculure, 142: 107-118.
Oettle E.E. (1982) Preliminary report: a pregnancy from frozen centrifuged dog semen. Journal of the South African Veterinary Association, 53: 269-270.
Park M.S., Kang C.K. and Lee P.Y. (2001) Reproductive cycle and biochemical composition of the ark shell Scapharca broughtonii (Schrenck) in a southern coastal bay of Korea. Journal of shellfish research, 20: 51-59.
Usuki H., M. Hamaguchi and H. Ishioka (1997) Long-term cryopreservation of Pacific oyster, Crassostrea gigas, sperm. Bulletin of Nansei National Fisheries Research Institute, 30: 115–123 [in Japanese with English abstracts]
Vuthiphandchai V., Thadsri I. and Nimrat S. (2009) Chilled storage of walking catfish (Clarias macrocephalus) semen. Aquaculture, 296(1-2): 58-64.
Yao Z., L.W. Crim, G.F. Richardson and C.J. Emerson (2000) Motility, fertility and ultrastructural changes of ocean pout (Macrozoarces americanus L.) sperm after cryopreservation. Aquaculture, 181(3-4):361-375.
Zilli L., R. Schiavone, V. Zonno, C. Storelli and S. Vilella (2003) Evaluation of DNA damage in Dicentrarchus labrax sperm following cryopreservation. Cryobiology, 47(3): 227-235.
이장희, 김인철 (1999) 돼지정자의 동결융해 후 활력 및 생존성에 대한 보존액, 동해보호제, 예비동결 및 동결처리시간의 영향. 한국동물번식학회지, 23: 165-174.
정정란, 유재규, 양성열, 여현진, 박종식 (2001) 개 정자의 보존 방법에 따른 첨체 및 생존성의 변화 II. 동결보존에 따른 효과. 한국수정란이식학회지, 16(2): 133-138.
