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This study was conducted to investigate the effects of multiple stresses on physiological parameters in the hepatopancreas and hemolymph of a Pacific abalone, Haliotis discus hannai. The alteration of antioxidant enzymes including catalase (CAT), superoxide dismuatase (SOD) and immunological indicators such as phenoloxidase (PO), lysozyme activity and total hemocyte count (THC) were measured in a Pacific abalone exposed to nickel chloride solution of sublethal concentrations (200 and 400 μg/L) under higher thermal conditions (22 and 26℃) for 2 weeks. In this study, the hepatopancreas enzymes, CAT and SOD activity were significantly increased in the Pacific abalone exposed to NiCl2 compared to those of the control condition(18℃) at both 22 and 26℃. PO activity in the Pacific abalone exposed to NiCl2 also significantly increased at 26℃ thermal stress. But, THC decreased in the Pacific abalone exposed to NiCl2 at 26℃ (P < 0.05). Lysozyme activity was markedly elevated at 22℃ for 1 weeks and at 18℃ for 2 weeks after NiCl2 exposure. Our results showed that NiCl2 concentrations upper 200 μg/L were able to stimulate immune responses in Pacific abalones. Furthermore, complex stress, thermal stress, or NiCl2 can modify the immunological response and can lead to changes in the physiology of host-pollutant interactions in H. discus hannai. From these results, these biochemical parameters may represent a convenient indicator of monitoring heavy metal pollution in coastal areas.
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