open access
메뉴ISSN : 1225-3480
In order to understand the gene expression of the Pacific oyster, Crassostrea gigas, during the early development stage of fertilized egg, transcriptome analysis was carried out to identify the embryogenesis-related genes from mRNA of each stage of embryo such as pre-fertilization-, fertilization membrane-, and polar body release-stage, respectively. Specifically expressed genes identified MYC, CDC25B, CCNB1, CLN3, DMC1, ADCY2, ADCY6, FBXO5 in prefertilization-stage, GRR1, CLB3_3, FBXO43 in fertilization membrane-stage, and BUB1B in polar body release-stage, respectively. A quantitative analysis of gene expression identified 6 candidates for embryogenesis regulation genes including PPP1CB, AUR, CDC25A, SLK, NCAPD2 and Hsp70 in the early stage of embryo development. These genes should be useful markers for further studies for gene regulation of polyploidy embryo development.
Aguilera, F. (2017) Neoplasia in mollusks: what does it tell us about cancer in humans?-a review. Journal of Genetic Disorders, 1: 07-16.
Allen Jr, S., and Tiews, K. (1987) Genetic manipulations:critical review of methods and performances for shellfish. In; Tiews K (ed) Symposium on Selection, Hybridization and Genetic Engineering in Aquaculture of Fish and Shellfish for Consumption and Stocking eng. Berlin (Germany, F.R.) Heenemann Verlagsgesellschaft Bordeaux (France).
Allen Jr, S.K., and Downing, S.L. (1986) Performance of triploid Pacific oysters, Crassostrea gigas (Thunberg). I. Survival, growth, glycogen content, and sexual maturation in yearlings. Journal of Experimental Marine Biology and Ecology, 102: 197-208.
An, L.-h., Zheng, B.-h., Liu, R.-z., Fan, Q., Wang, Q.-k., and Luo, Y.-f. (2014) Transcriptomic response to estrogen exposure in the male Zhikong scallop, Chlamys farreri. Marine pollution bulletin, 89: 59-66.
Barker, H.M., Brewis, N.D., Street, A.J., Spurr, N.K., and Cohen, P.T. (1994) Three genes for protein phosphatase 1 map to different human chromosomes:sequence, expression and gene localisation of protein serine/threonine phosphatase 1 beta (PPP1CB). Biochim Biophys Acta, 13: 212-218.
Beaumont, A. (1991) Ploidy manipulation in molluscan shellfish: a review. Journal of Shellfish Research, 10:1-18.
Beaumont, A.R., and Fairbrother, J.E. (1991) Ploidy manipulation in molluscan shellfish : a review. Journal of Shellfish Research, 10: 1-18.
Chaiton, J.A., and Allen Jr, S.K. (1985) Early detection of triploidy in the larvae of Pacific oysters, Crassostrea gigas, by flow cytometry. Aquaculture, 48: 35-43.
Conesa, A., Götz, S., García-Gómez, J.M., Terol, J., Talón, M., and Robles, M. (2005) Blast2GO: a universal tool for annotation, visualization and analysis in functional genomics research. Bioinformatics, 21: 3674-3676.
Cotterman, R., Jin, V.X., Krig, S.R., Lemen, J.M., Wey, A., Farnham, P.J., and Knoepfler, P.S. (2008) N-Myc regulates a widespread euchromatic program in the human genome partially independent of its role as a classical transcription factor. Cancer research, 68:9654-9662.
Dan, I., Watanabe, N.M., and Kusumi, A. (2001) The Ste20 group kinases as regulators of MAP kinase cascades. Trends in cell biology, 11: 220-230.
David, E., Tanguy, A., Pichavant, K., and Moraga, D. (2005) Response of the Pacific oyster Crassostrea gigas to hypoxia exposure under experimental conditions. The FEBS Journal., 272: 5635-5652.
Dheilly, N.M., Lelong, C., Huvet, A., and Favrel, P. (2011) Development of a Pacific oyster (Crassostrea gigas) 31,918-feature microarray: identification of reference genes and tissue-enriched expression patterns. BMC genomics, 12: 468.
Dheilly, N.M., Lelong, C., Huvet, A., Kellner, K., Dubos, M.-P., Riviere, G., Boudry, P., and Favrel, P. (2012)Gametogenesis in the Pacific Oyster Crassostrea gigas: A Microarrays-Based Analysis Identifies Sex and Stage Specific Genes. PLOS ONE, 7: e36353.
Ellis, R.J., and Van der Vies, S.M. (1991) Molecular chaperones. Annual review of biochemistry, 60:321-347.
Flick, J.S., and Johnston, M. (1991) GRR1 of Saccharomyces cerevisiae is required for glucose repression and encodes a protein with leucine-rich repeats. Molecular and Cellular Biology, 11:5101-5112.
Fu, X., Chen, G., Cai, Z.-D., Wang, C., Liu, Z.-Z., Lin, Z.-Y., Wu, Y.-D., Liang, Y.-X., Han, Z.-D., Liu, J.-C., and Zhong, W.-D. (2016) Overexpression of BUB1B contributes to progression of prostate cancer and predicts poor outcome in patients with prostate cancer. OncoTargets and therapy, 9: 2211-2220.
Galaktionov, K., Jessus, C., and Beach, D. (1995) Raf1interaction with Cdc25 phosphatase ties mitogenic signal transduction to cell cycle activation. Genes &Development, 9: 1046-1058.
Gething, M.-J., and Sambrook, J. (1992) Protein folding in the cell. Nature, 355: 33.
Goff, S.A., Vaughn, M., McKay, S., Lyons, E., Stapleton, A.E., Gessler, D., Matasci, N., Wang, L., Hanlon, M., and Lenards, A. (2011) The iPlant collaborative:cyberinfrastructure for plant biology. Frontiers in plant science, 2: 34.
Gui, L., and Homer, H. (2013) Hec1-dependent cyclin B2stabilization regulates the G2-M transition and early prometaphase in mouse oocytes. Developmental Cell, 25: 43-54.
Guo, X., and Allen Jr, S.K. (1994) Viable tetraploids in the Pacific oyster (Crassostrea gigas Thunberg)produced by inhibiting polar body 1 in eggs. Molecular Marine Biology and Biotechnology, 3:42-50.
Haas, B.J., Papanicolaou, A., Yassour, M., Grabherr, M., Blood, P.D., Bowden, J., Couger, M.B., Eccles, D., Li, B., Lieber, M., MacManes, M.D., Ott, M., Orvis, J., Pochet, N., Strozzi, F., Weeks, N., Westerman, R., William, T., Dewey, C.N., Henschel, R., LeDuc, R.D., Friedman, N., and Regev, A. (2013) De novo transcript sequence reconstruction from RNA-seq using the Trinity platform for reference generation and analysis. Nature Protocols, 8: 1494.
Habu, T., Taki, T., West, A., Nishimune, Y., and Morita, T. (1996) The mouse and human homologs of DMC1, the yeast meiosis-specific homologous recombination gene, have a common unique form of exon-skipped transcript in meiosis. Nucleic Acids Res, 24: 470-477.
Haidle, A.M., and Myers, A.G. (2004) An enantioselective, modular, and general route to the cytochalasins: Synthesis of L-696,474 and cytochalasin B. Proceedings of the National Academy of Sciences, 101: 12048-12053.
Hand, R., Nell, J., Reid, D., Smith, I., and Maguire, G. (1999) Studies on triploid oysters in Australia: effect of initial size on growth of diploid and triploid Sydney rock oysters, Saccostrea commercialis (Iredale & Roughley). Aquaculture research, 30: 34-42.
Helm, M.M. (2004) Hatchery culture of bivalves: a practical manual. FAO. Rome, 177 pp.
Huang, D.W., Sherman, B.T., and Lempicki, R.A. (2008)Bioinformatics enrichment tools: paths toward the comprehensive functional analysis of large gene lists. Nucleic acids research, 37: 1-13.
Korea, S. (2017) Fishery production survey. In; KOSTAT (ed). KOSIS, Daejeon.
Lara-Gonzalez, P., Westhorpe, F.G., and Taylor, S.S. (2012) The spindle assembly checkpoint. Current Biology, 22: 006.
Lehman, N.L., Verschuren, E.W., Hsu, J.Y., Cherry, A.M., and Jackson, P.K. (2006) Overexpression of the anaphase promoting complex/cyclosome inhibitor Emi1 leads to tetraploidy and genomic instability of p53-deficient cells. Cell Cycle, 5: 1569-1573.
Li, F.N., and Johnston, M. (1997) Grr1 of Saccharomyces cerevisiae is connected to the ubiquitin proteolysis machinery through Skp1: coupling glucose sensing to gene expression and the cell cycle. The EMBO journal., 16: 5629-5638.
Lutz, C.G. (2008) Practical genetics for aquaculture. Fishing News Books, Blackwell Science Ltd., Oxford, 256pp.
Ma, Y., Xie, N., Xie, D., Sun, L., Li, S., Li, P., Li, Y., Li, J., Dong, Z., and Xie, X. (2019) A novel homozygous FBXO43 mutation associated with male infertility and teratozoospermia in a consanguineous Chinese family. Fertility and Sterility, 111:909-917.e901.
Martin, C.-A., Murray, J.E., Carroll, P., Leitch, A., Mackenzie, K.J., Halachev, M., Fetit, A.E., Keith, C., Bicknell, L.S., and Fluteau, A. (2016) Mutations in genes encoding condensin complex proteins cause microcephaly through decatenation failure at mitosis. Genes & Development, 30: 2158-2172.
Martin, M. (2011) Cutadapt removes adapter sequences from high-throughput sequencing reads. EMBnet Journal, 17: 10-12.
Morgan, D.O. (1995) Principles of CDK regulation. Nature, 374: 131-134.
Mukherji, M., Bell, R., Supekova, L., Wang, Y., Orth, A.P., Batalov, S., Miraglia, L., Huesken, D., Lange, J., and Martin, C. (2006) Genome-wide functional analysis of human cell-cycle regulators. Proceedings of the National Academy of Sciences, 103: 14819-14824.
Nell, J.A. (2002) Farming triploid oysters. Aquaculture, 210: 69-88.
Nurse, P. (1990) Universal control mechanism regulating onset of M-phase. Nature, 344: 503.
Rime, H., Neant, I., Guerrier, P., and Ozon, R. (1989)6-Dimethylaminopurine (6-DMAP), a reversible inhibitor of the transition to metaphase during the first meiotic cell division of the mouse oocyte. Developmental biology, 133: 169-179.
Saskova, A., Solc, P., Baran, V., Kubelka, M., Schultz, R.M., and Motlik, J. (2008) Aurora kinase A controls meiosis I progression in mouse oocytes. Cell Cycle, 7:2368-2376.
Siah, A., McKenna, P., Danger, J.-M., Johnson, G.R., and Berthe, F.C.J. (2011) Induction of transposase and polyprotein RNA levels in disseminated neoplastic hemocytes of soft-shell clams: Mya arenaria. Developmental & Comparative Immunology, 35: 151-154.
Siah, A., McKenna, P., Danger, J.M., Johnson, G., and Berthe, F.C.J. (2012) Expression of RAS-like family members, c-jun and c-myc mRNA levels in neoplastic hemocytes of soft-shell clams Mya arenaria using microsphere-based 8-plex branched DNA assay. Results in Immunology, 2: 83-87.
Simão, F.A., Waterhouse, R.M., Ioannidis, P., Kriventseva, E.V., and Zdobnov, E.M. (2015) BUSCO:assessing genome assembly and annotation completeness with single-copy orthologs. Bioinformatics, 31: 3210-3212.
Stanley, J.G., Allen, S.K., and Hidu, H. (1981)Polyploidy induced in the American oyster, Crassostrea virginica, with cytochalasin B. Aquaculture, 23: 1-10.
Stanley, J.G., Hidu, H., and Allen Jr, S.K. (1984)Growth of American oysters increased by polyploidy induced by blocking meiosis I but not meiosis II. Aquaculture, 37: 147-155.
Tavaria, M., Gabriele, T., Kola, I., and Anderson, R.L. (1996) A hitchhiker's guide to the human Hsp70family. Cell Stress Chaperones, 1: 23-28.
Trapnell, C., Roberts, A., Goff, L., Pertea, G., Kim, D., Kelley, D.R., Pimentel, H., Salzberg, S.L., Rinn, J.L., and Pachter, L. (2012) Differential gene and transcript expression analysis of RNA-seq experiments with TopHat and Cufflinks. Nature Protocols, 7: 562.
Yang, W.L., Li, J., An, P., and Lei, A.M. (2014) CDC20downregulation impairs spindle morphology and causes reduced first polar body emission during bovine oocyte maturation. Theriogenology, 81:535-544.
Zhang, S., Li, J., Lea, R., Amaya, E., and Dorey, K. (2013) A Functional Genome-Wide In Vivo Screen Identifies New Regulators of Signalling Pathways during Early Xenopus Embryogenesis. PLOS ONE, 8:e79469.