바로가기메뉴

본문 바로가기 주메뉴 바로가기

ACOMS+ 및 학술지 리포지터리 설명회

  • 한국과학기술정보연구원(KISTI) 서울분원 대회의실(별관 3층)
  • 2024년 07월 03일(수) 13:30
사전등록 바로가기
 

logo

메뉴
search

ISSN : 1225-3480

논문 상세

이전 다음
논문 투고

36권 1호

멸종위기야생동물 I급 나팔고둥 (Charonia sauliae) 의 Aquaporin 유전자를 활용한 분자계통 분류학적 연구

Phylogenetic analysis of Charonia sauliae inferred from Aquaporin gene

정종민 (순천향대학교)
황희주 (순천향대학교)
상민규 (순천향대학교)
박지은 (순천향대학교)
송대권 (순천향대학교)
정준양 (순천향대학교)
조용훈 (전남대학교)
한연수 (전남대학교)
박홍석 (지앤시바이오)
이준상 (순천향대학교 자연과학대학 기초과학연구소)
신이현 (순천향대학교 자연과학대학 기초과학연구소)
박소영 (국립낙동강생물자원관 다양성연구팀)
이용석 (순천향대학교)
한국패류학회지 / The Korean Journal of Malacology, (P)1225-3480;
2020, v.36 no.1, pp.35-43
정종민, 황희주, 상민규, 박지은, 송대권, 정준양, 조용훈, 한연수, 박홍석, 이준상, 신이현, 박소영, & 이용석. (2020). 멸종위기야생동물 I급 나팔고둥 (Charonia sauliae) 의 Aquaporin 유전자를 활용한 분자계통 분류학적 연구. 한국패류학회지, 36(1), 35-43.

Abstract

Charonia sauliae, the largest sea snail is designated as the class I endangered species of red list data in Korea, because of habitat destruction and environmental pollution. For the conservation and restoration of this species, identification of genetic resources through transcriptomic and genomic studies, and investigation of ecological information are needed. In this study, we identified aquaporin (AQP) which plays an essential role in the water channel, by local-blast analysis with the nucleotide database of C. sauliae generated by Illumina Hi-Seq 2000 next-generation sequencing platform. AQP of C. sauliae contained 897 bp of nucleotides encoding 299 amino acid residues. The domain analysis of AQP showed one aquaporin-like domain including six transmembrane domains, eight amino acid residues which are related to the amphipathic channel, and two Asn-Pro-Ala signature motifs (NPA boxes). 3-Dimensional analysis indicated that it included six alpha-helix structures, which formed the transmembrane channel. To understand the evolutionary-relationship of AQP, conducted the multiple alignments and phylogenetic analysis, resulting in that AQP contained highly conserved NPA boxes and transmembrane domain. AQP of C. sauliae was localized in the same branch with Pomacea canaliculata (Ampullariidae). In the future, our results provide valuable information for the functional characterization of marine invertebrate aquaporin.

keywords
Charonia saulia, Aquaporun, Phylogenetics

참고문헌

1.

Abir-Awan, M., Kitchen, P., Salman, M.M., Conner, M.T., Conner, A.C., and Bill, R.M. (2019) Inhibitors of Mammalian Aquaporin Water Channels. International Journal of Molecular Sciences, 20(1589):1-22.

2.

Agre, P. (2006) The Aquaporin Water Channels. Proceedings of the American Thoracic Society, 3:5-15.

3.

Bang, I.S., Han, Y.S., Lee, J.-S., and Lee, Y.S. (2010)Current Status of Genome Research in Phylum Mollusks. The Korean Journal of Malacology, 26:317-326.

4.

Colgana, D.J., and Santosa, R.P. (2018) A phylogenetic classification of gastropod aquaporins. Marine Genomics, 38: 59-65.

5.

Haas, B.J., Papanicolaou, A., Yassour, M., Grabherr, M., Blood, P.D., Bowden, J., Couger, M.B., Eccles, D., Li, B., Lieber, M., MacManes, M.D., Ott, M., Orvis, J., Pochet, N., Strozzi, F., Weeks, N., Westerman, R., William, T., Dewey, C.N., Henschel, R., LeDuc, R.D., Friedman, N., and Regev, A. (2013) De novo transcript sequence reconstruction from RNA-seq using the Trinity platform for reference generation and analysis. Nat. Protoc., 8: 1494-1512.

6.

Jang, H.-Y., Lee, J.-E., Jang, Y.-S., and Ahn, S.-J. (2011)Conflicting Physiological Characteristics and Aquaporin (JcPIP2) Expression of Jatropha (Jatropha curcas L.) as a Bio-energy Crop under Salt and Drought Stresses. Korean Journal of Crop Science, 56: 183-191.

7.

Jin, J.-E., Kang, S.-H., Kim, B.-H., Lim, D.-J., Lee, C.-H., Oh, D.-H., Jung, M.-S., and Kim, H.-G. (2006)The Study of Distribution and Expression of Water Channel Proteins (Aquaporins) in the Rat Eustachian Tube. Korean Journal of Otology, 49: 598-603.

8.

Kang, S.W., Park, S.Y., Hwang, H.J., Chung, J.M., Sang, M.K., Min, H.R., Park, J.E., Cho, H.C., Patnaik, B.B., and Lee, Y.S. (2019) PANM DB ver 3.0 : An update of the bioinformatics database for annotation of large datasets from sequencing of species under Protostomia clade. The Korean Journal of Malacology, 35: 73-75.

9.

Kang, S.W., Park, S.Y., Patnaik, B.B., Hwang, H.J., Chung, J.M., Song, D.K., Park, Y.-S., Lee, J.S., Han, Y.S., Park, H.S., and Lee, Y.S. (2016) The Protostome database (PANM-DB): Version 2.0 release with updated sequences. The Korean Journal of Malacology, 32: 185-188.

10.

Kang, S.W., Park, S.Y., Patnaik, B.B., Hwang, H.J., Kim, C., Kim, S., Lee, J.S., Han, Y.S., and Lee, Y.S. (2015) Construction of PANM Database (Protostome DB) for rapid annotation of NGS data in Mollusks. The Korean Journal of Malacology, 31: 243-247.

11.

Kelley, L.A., Mezulis, S., Yates, C.M., Wass, M.N., and Sternberg, M.J.E. (2015) The Phyre2 web portal for protein modeling, prediction and analysis. Nature Protocol, 10(6): 845-858.

12.

Kumar, S., Stecher, G., Li, M., Knyaz, C., and Tamura, K. (2018) MEGA X: Molecular Evolutionary Genetics Analysis across Computing Platforms. Mol. Biol. Evol., 35: 1547-1549.

13.

Larkin, M.A., Blackshields, G., Brown, N.P., Chenna, R., McGettigan, P.A., McWilliam, H., Valentin, F., Wallace, I.M., Wilm, A., Lopez, R., Thompson, J.D., Gibson, T.J., and Higgins, D.G. (2007) Clustal W and Clustal X version 2.0. Bioinformatics, 23:2947-2948.

14.

Li, C., and Wang, W. (2017) Molecular Biology of Aquaporins. Advances in Experimental Medicine and Biology, 969: 1-34.

15.

Lu, H.A.J. (2017) Diabetes Insipidus. Advances in Experimental Medicine and Biology, 969: 213-225.

16.

Martin, M. Cutadapt removes adapter sequences from high-throughput sequencing reads. Technical Notes, 171: 10-12.

17.

Min Guo., Yeeting E. Chong., Ryan Shapiro., Kirk Beebe., Xiang-Lei Yang., and Paul Schimmel (2010)Paradox of mistranslation of serine for alanine caused by AlaRS recognition dilemma. Nature, 462:808-812.

18.

NA, J., and JN, F. (2011) Sickle: A sliding-window, adaptive, quality-based trimming tool for FastQ files (Version 1.33)[Software].

19.

Oh, D.-S., Lu, H., and Han, K.-H. (2011) Identification and Characterization of the Aquaporin Gene AQPA in a Filamentous Fungus Aspergillus nidulans. The Korean Journal of Microbiology, 47(4): 295-301.

20.

Park, N.C., Young Soo Park, Oh, G.S., and Jung, J.S. (2000) Molecular Identification and Distribution of Aquaporins in Human and Rat Testes. Korean Journal of Fertility and Sterility, 27: 133-144.

21.

Pertea, G., Huang, X., Liang, F., Antonescu, V., Sultana, R., Karamycheva, S., Lee, Y., White, J., Cheung, F., Parvizi, B., Tsai, J., and Quackenbush, J. (2003)TIGR Gene Indices clustering tools (TGICL):a software system for fast clustering of large ESTdatasets. Bioinfomatics application note, 19:651-652.

22.

Rice, P., Longden, I., and Bleasby, A. (2000) EMBOSS:The European Molecular Biology Open Software Suite. The European Molecular Biology Open Software Suite, 16(6): 276-277.

23.

Silva, I.V.d., and Soveral, G. (2017) Aquaporins in Obesity. Advances in Experimental Medicine and Biology, 969: 227-238.

24.

Verkman, A.S. (2017) Aquaporins at a glance. Journal of Cell Science, 124: 2107-2112.

25.

강경호, 황성일, 김재민, 임상민, Brozozowska, R., 선승천, LiangLiang, Z., 박용빈, 김현중, 김창봉, 이재영, 허은경와 노평우. (2009) 나팔고둥의 대량 인공종묘생산 기법 개발연구보고서. 188pp. 수산연구개발사업

26.

국립생물자원관 (2012) 한국의 멸종위기 야생동·식물 적색자료집 (연체동물) 107pp. 국립생물자원관.

27.

김수완, 이종은, 염충호, 나명윤, 김남호 와 최기철. (2001) 신장에서 Aquaporin 수분 통로의 생리적 및 병태생리적 기능. 대한신장학회지, 20(5): 785-801.

28.

방상원 와 조미경. (2008) 친환경농법용 외래 왕우렁이의 생태위해성 및 위해성 관리의 필요성. Korean Journal of Environment Biology, 26(3): 129-137.

29.

이준상 와 민덕기. (2002) 한국산 연체동물 목록. Korean Journal of Malacology, 18(2): 93-217.

30.

조혜정, 이창배 와 안규윤. (2008) 임신 흰쥐 신장에서Aquaporin-1, 2, 3 단백발현의 변화. The Korean Journal of Anatomy, 41(4): 289-296.

logo

논문 투고
OA 정책