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- E-ISSN 2288-8985
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Development of New Analytical Method of Vitamins Using Supercritical Fluid
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
1997, v.10 no.2, pp.131-138
Pyo,
D., Park,
D., Kim,
H., Lee,
H., &
Lee,
T.
(1997). Development of New Analytical Method of Vitamins Using Supercritical Fluid. Analytical Science and Technology, 10(2), 131-138.
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Abstract
Supercritical Fluid Chromatography(SFC) has become a technique for solving problems that are difficult to be monitored by other chromatographic methods. However, the most widely used fluid, is no more polar than hexane. Polar samples which are difficult to be analyzed with pure supercritical <TEX>$CO_2$</TEX> because of their high polarity can be separated by adding polar modifiers to supercritical <TEX>$CO_2$</TEX>. In this paper, a new method for monitoring the mobile phase composition in modified supercritical fluid chromatography was developed. The amount of water dissolved in supercritical <TEX>$CO_2$</TEX> was measured by amperometric microsensor which is made of thin film of perfluorosulfonate ionomer(PFSI). The amount of water dissolved in supercritical <TEX>$CO_2$</TEX> stayed constant for a much longer time than with a saturator column. With this new mixing device, we could get good resolutions for vitamins which are difficult to separate with pure <TEX>$CO_2$</TEX>.
- keywords
- Supercritical Fluid Chromatography, vitamins, modifier
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