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Separation and Activity Test of Antifungal Substance from C. japonica Extract
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
1997, v.10 no.2, pp.153-159
Chung,
I., &
Paik,
S.
(1997). Separation and Activity Test of Antifungal Substance from C. japonica Extract. Analytical Science and Technology, 10(2), 153-159.
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Abstract
This study was conducted to test antifungal activity and to separate bioactive compound using C. japonica extract which was shown the most effective control on the B. berengeriana, G. cingulata and P. expansum. <TEX>$EC_{50}$</TEX> on the test pathogene of C. japonica extract was <TEX>$400{\sim}500{\mu}g/mL$</TEX>. Bioactive compound analyzed by TLC and HPLC method was identified as a kind of alkaloids, berberine, and the content was 81.14%.
- keywords
- Coptis japonica, antifungal activity, bioactive compound, HPLC, berberine
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