Abstract

Scutellariae radix has been used on the control of body fever as oriental medicine for thousand years. Analytical aspect for the main components of Scutellariae radix was set up improving sensitivity and resolution. The analysis of 3 different flavonoids present in Scutellariae radix-baicalin, baicalein, wogonin-was conducted by means of high performance liquid chromatography with ODS reverse phase column in conjunction with a Photo Diode Array UV detector(280nm) at <TEX>$40^{\circ}C$</TEX>. Mobile phase was carried out at 1mL/min, composed of acetonitrile and 0.1M phosphoric acid in the form of a gradient method. Under these circumstances the retention time for baicalin, baicalein, wogonin was 7.65, 11.65 and 14.12 minutes respectively. As a result for the efficiency on extraction of active ingredients with proposed analytical process according to it's growing districts, Sunchang in Junbuk for baicalin and Bulkyo in, Junnam for bicalein and wogonin have shown the best results. Even the extraction at room temperature was satisfactory. Among acids, 0.1M acetic acid revealed the best achievements. The mixture of acetonitrile and 0.2M phosphoric acid(75:25) has been shown the best efficiency as well as stability for the extraction of active ingredients.