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  • P-ISSN 1225-0163
  • E-ISSN 2288-8985

Analysis of Kasugamycin in Pesticide Formulations by Reversed-Phase Ion Pair Liquid Chromatography

Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
1997, v.10 no.5, pp.343-349
Kim, Taek-Jae
Kim, Kyong-Sun
Yoon, Chae-Hyuk
Joo, Jin-Bok
Kim, Chung-Hyo
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Abstract

Analytical method using the reversed phase ion-pair chromatography (RP-IPC) for the determination of kasugamycin(5-amino-2-methyl-6-(2,3,4,5,6-pentahydroxy cyclohexyloxy)tetrahydropyran-3-yl-amino-<TEX>${\alpha}$</TEX>-imino acetic acid), pesticide as fungicide bactercide has been established. The retention behavior of kasugamycin in the RP-IPC was examined with respect to the effect of concentrations of organic modifiers, pH of eluent and types and concentrations of the counter ions as ion-pair reagent. This method developed by the optimum factors, can be used for the application of the quality control in the crude product and its formulation.

keywords
reversed phase, ion-pair, HPLC, kasugamycin


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