- P-ISSN 1225-0163
- E-ISSN 2288-8985
- Log In/Sign Up
- P-ISSN1225-0163
- E-ISSN2288-8985
- SCOPUS, ESCI, KCI
Article Detail
Home > Article Detail
Functional analysis of Tyr7 residue in human glutathione S-transferase P1-1
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
1997, v.10 no.5, pp.378-385
Kong,
K., Park,
H., Yoon,
S., &
Cho,
S.
(1997). Functional analysis of Tyr7 residue in human glutathione S-transferase P1-1. Analytical Science and Technology, 10(5), 378-385.
- Downloaded
- Viewed
Abstract
In order to clarify the functional role of Tyr7 in human glutathione S-transferase P1-1, we extensively investigated the effect of mutation of Tyr7 on the substrate specificity and inhibition characteristics. The mutational replacement of Tyr7 with phenylalanine lowered the specific activities with 1,2-dichloro-4-nitrobenzene and 1,2-epoxy-3-(p-nitrophenoxy) propane for GSH-conjugation reaction to 3~5% of the values for the wild-type enzyme. The pKa of the thiol group of GSH bound in Y7F was about 2.4 pK units higher than that in the wild-type enzyme. The <TEX>$I_{50}$</TEX> of hematin for Y7F was similar to that for the wild-type enzyme and those of benastatin A and S-(2,4-dinitrophenyl)glutathione were only moderately decreased. These results suggest that Tyr7 is considered to be important the catalytic activities not only for GSH-chloronitrobenzene derivatives but also for GSH-epoxide conjugation reaction, rather than to binding of the substrates.
- keywords
- glutathione S-tranferase, tyrosine 7 residue, active site, substrate spedficity, inhibition characteristics
- Downloaded
- Viewed
- 0KCI Citations
- 0WOS Citations
Other articles from this issue
- A Sequential Separation Procedure for the Determination of Uranium, Thorium and Their Isotopes in Geological Materials
- A Study on the Analysis of Polycyclic Aromatic Hydrocarbons by RPLC/DAD (I)
- Improved Separation of Organic Explosives by Modified Micellar Electrokinetic Capillary Chromatography
- Determination of Organic Pollutants in Dyeing Wastewater
- Analysis of Kasugamycin in Pesticide Formulations by Reversed-Phase Ion Pair Liquid Chromatography
- Development of New Separation Technique, Modifier Composition Programming in Supercritical Fluid Chromatography
- Analysis of multi-layered Surface with Glow Discharge-Mass Spectrometry
- Chemometric Aspects and Determination of Sugar Composition of Honey by HPLC
- Extraction Equilibria and Analytical Application of Metal-Dithizone Complexes(II) Partition and Extraction Equilibria into Chloroform
- Functional analysis of Tyr7 residue in human glutathione S-transferase P1-1
- Evaluation of Quantitative Results of PIXE Analysis
- Surface Analysis by Time-Of-Flight Secondary Ion Mass Spectrometry(TOF-SIMS)
- more
Recommanded Articles
This website recommended the use the Chrome browser for journal website.