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- E-ISSN 2288-8985
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Analusis of Endotoxin Using Analtical Conditions of Optical Density in Metalworking Fluid Sample
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
2002, v.15 no.5, pp.459-465
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(2002). Analusis of Endotoxin Using Analtical Conditions of Optical Density in Metalworking Fluid Sample. Analytical Science and Technology, 15(5), 459-465.
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Abstract
This study was performed to identify the proper analytical conditions of endotoxins regarding optical density in the workplace where metalworking fluids are used. This study found that "onset time method" was more accurate than "time to <TEX>$V_{max}$</TEX> method". Reproducibility and accuracy analyzed by "onset time method" was greatly higher than the "time to <TEX>$V_{max}$</TEX>". The optical density of "0.03" was the most appropriate analytical condition among "onset time method". In this analytical condition, linearity of 0.998 was obtained and recovery rate ranged from 88% to 105% at the endotoxin concentrations below 5 EU/mL. No significant difference of endotoxins was observed between the optical densities of "0.03" and "0.05". However, correlation coefficients were different with statistical significance (p<0.01). This study confirmed that either the optical densities "0.03" or "0.05" should be used to analyze endotoxin. Of these optical density values, OD with correlation coefficient higher than 0.98 should be used to analyze endotoxin in environmental samples.
- keywords
- Metalworking fluids, endotoxin, optical density, Onset time method, time to <TEX>$V_{max}$</TEX>
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