- P-ISSN 1225-0163
- E-ISSN 2288-8985
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- P-ISSN1225-0163
- E-ISSN2288-8985
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Article Detail
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Separation and purification of substance having matrix metalloproteinase-9 inhibition effect in Ulmus davidiana Plancn. Var. japonica Nkai.
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
2003, v.16 no.3, pp.179-184
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(2003). Separation and purification of substance having matrix metalloproteinase-9 inhibition effect in Ulmus davidiana Plancn. Var. japonica Nkai.. Analytical Science and Technology, 16(3), 179-184.
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Abstract
Root bark of Ulmus davidiana Plancn. var. japonica Nakai was extracted by using several solvents with different polarities. Each extract was treated on the MMPs obtained from SK-Hep-1 in order to investigate inhibition effect. Zymography of MMPs showed that MeOH extract has significant inhibition effect. On the GC-MS analysis the highest mass to charge ratio (m/z) of the purified substance was 281. Also, on zymography of MMPs the substance showed 47% inhibition effect at the concentration of <TEX>$314.7{\mu}g/g$</TEX>. Cell viability of SK-Hep-1 was 60% at <TEX>$31.47{\mu}g/g$</TEX>.
- keywords
- Zymography, MMPs, GC-MS
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