- P-ISSN 1225-0163
- E-ISSN 2288-8985
- Log In/Sign Up
- P-ISSN1225-0163
- E-ISSN2288-8985
- SCOPUS, ESCI, KCI
Article Detail
Home > Article Detail
Molecular Size Fractionation of Soil Fulvic Acid by Gel Filtration Chromatography and Analysis of Their Fluorescencce Characteristics
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
2004, v.17 no.2, pp.163-172
&
(2004). Molecular Size Fractionation of Soil Fulvic Acid by Gel Filtration Chromatography and Analysis of Their Fluorescencce Characteristics. Analytical Science and Technology, 17(2), 163-172.
- Downloaded
- Viewed
Abstract
The molecular size distribution and fluorescence properties of soil fulvic acids (FA) were characterized by using gel filtration chromatography (GFC) and luminescence spectroscopy. The objectives of this work were to fractionate the FA extracted from a forest soil into different nominal molecular size using GFC system and to characterize the fluorescence properties (excitation, emission and synchronous) of these fractions using luminescence spectrometer. The GFC column was calibrated with polyethylene glycols, acetone and dextrane Blue. The total permeation volume of the GFC system was 404 mL and the void volume 130 mL. The GFC molecular weight of the soil FA was in the range of 190∼8,900 Dalton and the molecular weight at the peak on the chromatogram was 930 Dalton. The fluorescence intensity ratio (I498nm/I392nm) was found to be increased with an increasing molecular weight. This results may suggest that the fulvic acid fractions with high molecular weight have large amount of the condensed aromatic compound.
- keywords
- fulvic acids, GFC, molecular size fractionation, luminescence spectroscopy, fluorescence
Reference
1
(1985.) Geochemistry of Soil Humic Substances, in Humic Substances in Soil, Sed-iment and Water,
2
(2002) Development of Monitoring Technology for Environmental Radioactivity" KAERI/RR-2344/ 2002,
3
Isolation of IHSS soil fulvic and humic acids. , International Humic Substances Society,
Other articles from this issue
- Adsorption of Uranium(Ⅵ) Ion Wtilizing Cryptand Ion Exchange Resin
- Analytical Study for and Acrylic Coating
- A Study on the Adsorption of Rare Earth Elements by Raq and Crosslinked Chiosan.
- Quantitative Analysis and Qualification of Amitrole Using LC/ESI-MS
- The study on the measurement of volatile organic compounds in the air of A and B industrial area
- Studies of gas chromatographic analysis malodorous S compounds in air
- Preparation of CRM for QA/QC in dioxin analysis and inter-laboratory study
- Molecular Size Fractionation of Soil Fulvic Acid by Gel Filtration Chromatography and Analysis of Their Fluorescencce Characteristics
- Preparation and Characterization of BaTiO3 Powders and Thin films
- Determination of Histidene by Spectrofluorometry
- Comparison of Dioxin Analytical Result Between SP-2331 and DB-5MS Capillary columns in the HRGC/HRMS Anlaysis
- Development of the Discrimination Methods for Grographical Origin of Braken(Preridium aquilinum) by Capillary Electrophoresis
- more
Recommanded Articles
This website recommended the use the Chrome browser for journal website.