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Development of the Discrimination Methods for Grographical Origin of Braken(Preridium aquilinum) by Capillary Electrophoresis
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
2004, v.17 no.2, pp.192-197
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(2004). Development of the Discrimination Methods for Grographical Origin of Braken(Preridium aquilinum) by Capillary Electrophoresis. Analytical Science and Technology, 17(2), 192-197.
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Abstract
The discrimination of bracken (Korean vs. Chinese) has been attempted using capillary electrophoresis (CE). Bracken (Pteridium aquilinum) was extracted with 30% methanol and separated on a uncoated fused-silica (<TEX>$50{\mu}m{\times}27cm$</TEX>) capillary. Conditions for optimal analysis include: temperature, <TEX>$40^{\circ}C$</TEX>; voltage, 8 kV; and pressure injection time, 5 sec. The optimal separation buffer was 0.3 M borate buffer (pH 8.5) containing 40 mM CHAPS with 30% ethylene glycol. Under the optimal conditions established for CE, the ratio of specific peak area (peak PA-1) to other peak area (peak PA-2) was effective in discrimination of Korean and Chinese bracken. The mean accuracy for discrimination of Korean and Chinese brackens were 80% and 86%, respectively.
- keywords
- bracken, capillary electrophoresis, conditions for optimal analysis, discrimination
Reference
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