- P-ISSN 1225-0163
- E-ISSN 2288-8985
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- P-ISSN1225-0163
- E-ISSN2288-8985
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Determination of Histidene by Spectrofluorometry
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
2004, v.17 no.2, pp.180-183
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(2004). Determination of Histidene by Spectrofluorometry. Analytical Science and Technology, 17(2), 180-183.
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Abstract
A simple and sensitive determination method of histidine has been investigated using the spectrofluorometry. An emission peak of Eu3+ - TTA- histidine complex occurs at 470 nm in aquous solution with excitation at 235 nm and the fluorescence intensity increased linearly with addition of histidine in the range of 1 x 10-7 - 4 x 10-6 M. The detection limits was 5 x 10-7 M with 3.5% RSD. The present method was applied to determine histidine content in the synthetic sample.
- keywords
- Histidine, Amino acids, <TEX>$Eu^{3+}$</TEX>, TTA(thenoyltrifluoroacetone), EDTA(ethylenediaminetetracetic acid)
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