ACOMS+ 및 학술지 리포지터리 설명회

  • 한국과학기술정보연구원(KISTI) 서울분원 대회의실(별관 3층)
  • 2024년 07월 03일(수) 13:30
 

  • P-ISSN1225-0163
  • E-ISSN2288-8985
  • SCOPUS, ESCI, KCI

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  • P-ISSN 1225-0163
  • E-ISSN 2288-8985

Capillary electrophoresis를 이용한 한국산 및 중국산 고사리의 원산지 판별방법 개발

Development of the Discrimination Methods for Grographical Origin of Braken(Preridium aquilinum) by Capillary Electrophoresis

분석과학 / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
2004, v.17 no.2, pp.192-197
류미라 (한국식품개발연구원)
김은영 (한국식품개발연구원)
김정현 (한국식품개발연구원)
정경숙 (한국식품개발연구원)
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Abstract

The discrimination of bracken (Korean vs. Chinese) has been attempted using capillary electrophoresis (CE). Bracken (Pteridium aquilinum) was extracted with 30% methanol and separated on a uncoated fused-silica (<TEX>$50{\mu}m{\times}27cm$</TEX>) capillary. Conditions for optimal analysis include: temperature, <TEX>$40^{\circ}C$</TEX>; voltage, 8 kV; and pressure injection time, 5 sec. The optimal separation buffer was 0.3 M borate buffer (pH 8.5) containing 40 mM CHAPS with 30% ethylene glycol. Under the optimal conditions established for CE, the ratio of specific peak area (peak PA-1) to other peak area (peak PA-2) was effective in discrimination of Korean and Chinese bracken. The mean accuracy for discrimination of Korean and Chinese brackens were 80% and 86%, respectively.

keywords
bracken, capillary electrophoresis, conditions for optimal analysis, discrimination


참고문헌

1

(1991) J. Chromatogr,

2

(1992) Cereal Foods World,

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