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- E-ISSN 2288-8985
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Mixed Bacillus sp. BOD sensor
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
2007, v.20 no.1, pp.1-9
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(2007). Mixed Bacillus sp. BOD sensor. Analytical Science and Technology, 20(1), 1-9.
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Abstract
The BOD (biochemical oxygen demand) sensor was fabricated by covering a dissolved oxygen (DO) probe with a microbe-impregnated membrane and a dialysis membrane. Various microorganisms isolated from the soils, water and activated sludge have been evaluated for measuring biochemical oxygen demand (BOD); Bacillus species HN24 and HN93 were selected as they exhibited rapid oxygen consumption and fast recovery. Improved BOD sensor could be prepared by using mixed microbes (Bacillus subtilis, Bacillus sp. HN24 and Bacillus sp. NH93) and silicon rubber gas-permeable membrane for DO probe, and by bubbling 50% <TEX>$O_2$</TEX> (<TEX>$N_2$</TEX> valence) through background buffer solution. This system exhibited excellent analytical performance resulting in good linearity (<TEX>$r^2
- keywords
- BOD sensor, microbial membrane, dissolved oxygen, Bacillus sp.
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