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- E-ISSN 2288-8985
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Development of electrochemical biosensor for determination of galactose
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
2007, v.20 no.5, pp.393-399
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(2007). Development of electrochemical biosensor for determination of galactose. Analytical Science and Technology, 20(5), 393-399.
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Abstract
In principle, the blood galactose level may be determined conveniently with a strip-type biosensor similar to that for glucose. In this study, we describe the development of a disposable galactose biosensor strip for point-of-care testing. The sensor strip is constructed with screen-printed carbon paste electrode (SPCE) and sample amount (< <TEX>$100{\mu}L$</TEX>). The developed strip the galactose level in less than 90 s using bienzymatic system of galactose oxidase (GAO) and horseradish peroxidase (HRP). The effects of pH, mediator (1,1-ferrocenedimethanol) concentration, ratio of enzymes, and applied potential were determined preliminarily with glassy carbon electrodes, and optimized further with the strip-type electrodes. The sensor exhibits linear response in the range of <TEX>$0{\sim}400{\mu}M$</TEX> (<TEX>$r^2$</TEX>
- keywords
- galactose biosensor, galactosemia, screen-printed carbon paste electrodes, point-of-care testing, galactose oxidase, horseradish peroxidase
Reference
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