- P-ISSN 1225-0163
- E-ISSN 2288-8985
- P-ISSN1225-0163
- E-ISSN2288-8985
- SCOPUS, ESCI, KCI
Article Detail
Home > Article Detail
Simultaneous determination of amphetamine-like drugs in human urine by SPE and GC/MS
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
2008, v.21 no.1, pp.41-47
&
(2008). Simultaneous determination of amphetamine-like drugs in human urine by SPE and GC/MS. Analytical Science and Technology, 21(1), 41-47.
- Downloaded
- Viewed
Abstract
소변 중 암페타민계 5성분(amphetamine; AP, methamphetamine; MA, 3,4-methylenedioxyamphetamine; MDA, 3,4-methylenedioxymethamphetamine; MDMA, 3,4-methylenedioxyethylamphetamine; MDEA) 의 동시분석을 위하여 기존의 액체상추출(liquid-liquid extraction; LLE) 방법과는 달리 감정과정의 자동 화가 가능한 고체상추출(solid-phase extraction, SPE) 방법을 도입하였다. 시험관에 소변 3 mL와 0.1 M 인산완충액 1 mL (pH 7.0)를 넣고, 자동추출장치를 이용하여 추출하고, 증발·건고하여 유도체화 한 다 음 GC/MS로 분석하였다. 그 결과 검정곡선의 직선성 상관계수 (r2)는 AP와 MDA [농도범위 34.0 (AP), 28.0 (MDA)~1000.0 ng/mL] 및 MA, MDMA, MDEA (농도범위 50.0~2000.0 ng/mL)에서 0.994 이상으로 나타났다. 그리고 각 성분들의 검출한계 (LOD)는 4.0~10.0 ng/mL 범위였고, 정량한계 (LOQ)는 12.0~34.0 ng/mL 범위였다. 상대 회수율은 5성분 모두 93.5~107.7%로 측정되었다. 정밀도 (precision)와 정확도 (accuracy)는 각각 1.9~14.8%와 -8.7~14.8 % 범위 값을 나타냈다. 본 실험방법을 실제 남용자 소변에 적 용한 결과 메스암페타민 또는 엑스터시 투약자를 신속하고 정확하게 동시에 확인할 수 있었다.
- keywords
- Methamphetamine, Ecstasy (MDMA), SPE, GC/MS, urine
Reference
1
1. G. W. Kunsman, B. Levine, J. J. Kuhlman, R. L. Jones,R. O. Hughes, C. I. Fujiyama, and M. L. Smith, J.Anal. Toxicol., 20(7), 517-521(1996).
2
2. J. K. Fallon, A. T. Kicman, J. A. Henry, P. J. Milligan,D. A. Cowan, and A. J. Hutt, Clin. Chem., 45(7), 1058-1069(1999).
3
3. 김진영, 서승일, 고범준, 이재일, 정재철, 서용준, 인문교, 약학회지, 47(3), 142-147(2003).
4
4. G. W. Kunsman, J. E. Manno, K. R. Cockerham, and B.R. Manno, J. Anal. Toxicol., 14(3), 149-153(1990).
5
5. Z. Huang and S. Zhang, J. Chromatogr. B., 792(2), 241-247(2003).
6
6. 대검찰청, “마약류범죄백서”, 106-108, 2006.
7
7. C. E. Cook, A. R. Jeffcoat, J. M. Hill, D. E. Pugh, P. K.Patetta, B. M. Sadler, W. R. White, and M. Perez-Reyes, Drug Metab. Dispos., 21(4), 717-723(1993).
8
8. H. H. Maurer, Ther. Drug Monit., 18(4), 465-470(1996).
9
9. H. K. Ensslin, K. A. Kovar, and H. H. Maurer, J. Chromatogr.B., 683(2), 189-197(1996).
10
10. M. Vaddevenne, H. Vandenbussche, and A. Verstraete,Acta Clin. Belgica, 55(6), 323-333(2000).
11
11. M. R. Lee, S. C. Yu, C. L. Lin, and Y. C. Yeh, J. Anal.Toxicol., 21(4), 278-282(1997).
12
12. B. K. Gan, D. Baugh, R. H. Liu, and A. S. Walia, J.Forensic. Sci., 36(5), 1331-1341(1991).
13
13. C. Jurado, M. P. Gimenez, T. Soriano, M. Menendez, and M. Repetto, J. Anal. Toxicol., 24(1), 11-16(2000).
14
14. P. R. Stout, C. K. Horn, and K. L. Klette, J. Anal. Toxicol.,26(5), 253-261(2002).
15
15. K. L. Klette, M. H. Jamerson, C. L. Morris-Kukoski, A.R. Kettle, and J. J. Snyder, J. Anal. Toxicol., 29(7),669-674(2005).
16
16. K. L. Klette, A. R. Kettle, and M. H. Jamerson, J. Anal.Toxicol., 30(5), 319-322(2006).
17
17. S. O. Pirnay, T. T. Abraham, and M. A. Huestis, Clin.Chem., 52(9), 1728-1734(2006).
18
18. G. W. kunsman, B. Levine, J. J. Kuhlman, R. L. Jones,R. O. Hughes, C. I. Fujiyama, and M. L. Smith, J. Anal.Toxicol., 20(7), 517-521(1996).
19
19. I. Kim, J. M. Oyler, E. T. Moolchan, E. J. Cone, and M.A. Huestis, Ther. Drug. Monit., 26(6), 664-672(2004).
Other articles from this issue
- Characteristics of the release of chromium, copper, and arsenic from CCA-treated wood exposed to the natural environment
- Distribution of CO2 produced from fossil fuel by accelerator mass spectrometry: in Daejeon
- Development of certified reference materials for odorous aldehyde
- Determination of vitamin P1 by chemiluminescence method
- Preparation and characterization of prospective disulfide based electrode materials ofr lithium batteries
- Determination of lercanidipine in human plasma by LC-MS/MS
- Simultaneous determination of amphetamine-like drugs in human urine by SPE and GC/MS
- Validation of new saliva test using SALIgAE®
- Analysis of ethyl carbamate in alcoholic beverages
- Characterization of TMA-A zeolite incorporated by ZnO nanocrystals
- more
Recommanded Articles
This website recommended the use the Chrome browser for journal website.