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- E-ISSN 2288-8985
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Simultaneous determination of amphetamine-like drugs in human urine by SPE and GC/MS
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
2008, v.21 no.1, pp.41-47
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(2008). Simultaneous determination of amphetamine-like drugs in human urine by SPE and GC/MS. Analytical Science and Technology, 21(1), 41-47.
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Abstract
소변 중 암페타민계 5성분(amphetamine; AP, methamphetamine; MA, 3,4-methylenedioxyamphetamine; MDA, 3,4-methylenedioxymethamphetamine; MDMA, 3,4-methylenedioxyethylamphetamine; MDEA) 의 동시분석을 위하여 기존의 액체상추출(liquid-liquid extraction; LLE) 방법과는 달리 감정과정의 자동 화가 가능한 고체상추출(solid-phase extraction, SPE) 방법을 도입하였다. 시험관에 소변 3 mL와 0.1 M 인산완충액 1 mL (pH 7.0)를 넣고, 자동추출장치를 이용하여 추출하고, 증발·건고하여 유도체화 한 다 음 GC/MS로 분석하였다. 그 결과 검정곡선의 직선성 상관계수 (r2)는 AP와 MDA [농도범위 34.0 (AP), 28.0 (MDA)~1000.0 ng/mL] 및 MA, MDMA, MDEA (농도범위 50.0~2000.0 ng/mL)에서 0.994 이상으로 나타났다. 그리고 각 성분들의 검출한계 (LOD)는 4.0~10.0 ng/mL 범위였고, 정량한계 (LOQ)는 12.0~34.0 ng/mL 범위였다. 상대 회수율은 5성분 모두 93.5~107.7%로 측정되었다. 정밀도 (precision)와 정확도 (accuracy)는 각각 1.9~14.8%와 -8.7~14.8 % 범위 값을 나타냈다. 본 실험방법을 실제 남용자 소변에 적 용한 결과 메스암페타민 또는 엑스터시 투약자를 신속하고 정확하게 동시에 확인할 수 있었다.
- keywords
- Methamphetamine, Ecstasy (MDMA), SPE, GC/MS, urine
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