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- E-ISSN 2288-8985
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- P-ISSN1225-0163
- E-ISSN2288-8985
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Evaluation of two DNA extraction methods on exhumed bone samples:Ultrafiltration versus column affinity
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
2008, v.21 no.4, pp.338-343
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(2008). Evaluation of two DNA extraction methods on exhumed bone samples:Ultrafiltration versus column affinity. Analytical Science and Technology, 21(4), 338-343.
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Abstract
발굴된 유골에서 DNA 추출은 DNA신원확인과정에서 매우 중요한 단계이지만 유골에 오염된 미 생물 DNA와 사람 DNA가 동시에 추출되는 문제점이 있다. 이를 극복하기 위해 발굴된 10명의 유골 시 료를 페놀-ultrafiltration 후 QIAquick® PCR Purification Kit (ultrafiltration법)와 QIAamp® DNA Mini kit(Column-affinity법)를 적용하여 전체(미생물+사람) DNA정량 및 사람 DNA정량 후 각각 STR마커를 분 석하므로서 DNA 분리 효율성을 확인하였다. 회수된 전체 DNA양은 Column-affinity법(19.6 ng/μL)이 ultrafiltration법(16.0 ng/μL) 보다 1.2배 더 나은 결과를 보였으나 사람 DNA 정량 결과로는 Column-affinity 법(0.034 ng/μL) 보다 ultrafiltration법(0.498 ng/μL)이 14.6배 더 많은 회수율을 나타냈다. 유골에 있던 다 량의 미생물 DNA가 사람 DNA와 섞여있을 경우, 사람 DNA가 실리카 친화성 컬럼에 부착되는 것이 미 생물 DNA의 영향을 받으나, 필터의 경우는 미생물 DNA의 영향을 받지 않아서 ultrafiltration법이 높은 회 수율을 보였다.
- keywords
- forensic sciences, DNA extraction, bone, microbial DNA contamination
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