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- E-ISSN 2288-8985
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Quantification of cholesterol in human serum by isotope dilution liquid chromatography/mass spectrometry
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
2008, v.21 no.6, pp.502-509
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(2008). Quantification of cholesterol in human serum by isotope dilution liquid chromatography/mass spectrometry. Analytical Science and Technology, 21(6), 502-509.
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Abstract
혈청 내 콜레스테롤을 정량분석하기 위한 일차분석법으로 동위원소희석 액체크로마토그래피/질 량분석법(isotope dilution liquid chromatography/mass spectrometry)을 사용하였다. 콜레스테롤은 Thermo ODS hypersil C18 칼럼을 사용하여 분리하였고, 이동상은 100% 메탄올, 유속은 0.3 mL/min으로 하였다. 콜레스테롤과 콜레스테롤-3,4-13C2는 [M-H2O+H]+이온에 해당하는 m/z 369.4와 371.3에서 모니터링하여 정량에 합당한 크로마토그램을 얻을 수 있었다. 방법의 유효성을 증명하기 위해서 NIST SRM 909b를 분 석한 결과 인증값과 불확도 범위 내에서 일치하는 것을 확인하였다. 이 방법을 바탕으로 혈청 인증표준 물질 4 종류를 제조하여 인증을 실시하였다. 인증 결과, 반복성의 상대표준오차는 0.1~0.8%, 재현성의 경 우 0.24%이하, 확장 불확도는 95% 신뢰도구간에서 약 1.43%로 나타났다.
- keywords
- cholesterol, ID LC/MS, primary method, traceability
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