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  • P-ISSN 1225-0163
  • E-ISSN 2288-8985

Electrochemical determination of hydrogen peroxide using carbon paste biosensor bound with butadiene rubber

Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
2010, v.23 no.5, pp.505-510
https://doi.org/10.5806/AST.2010.23.5.505

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Abstract

When polybutadiene dissolved in toluene was a binder of carbon powder, the volatility of solvent just after electrode fabrication assured the mechanical solidity of the carbon paste electrode. This characteristic met the qualifications for practical use of carbon paste electrodes. A new enzyme electrode bound with butadiene rubber was constructed. In order to confirm whether it shows quantitative electrochemical behaviors or not,its electrochemical kinetic parameters, e.g. the symmetry factor, the exchange current density, the capacitance of double layer, the time constant, the maximum current, the Michaelis constant and other factors were investigated. These experimental facts showed that butadiene rubber is a recommendable binder for practical use of a carbon paste electrode.

keywords
carbon paste electrode, hydrogen peroxide, peroxidase, butadiene rubber


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