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- E-ISSN 2288-8985
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Determination of finasteride in human serum by LC-MS/MS
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
2011, v.24 no.5, pp.345-351
https://doi.org/10.5806/AST.2011.24.5.345
&
(2011). Determination of finasteride in human serum by LC-MS/MS. Analytical Science and Technology, 24(5), 345-351, https://doi.org/10.5806/AST.2011.24.5.345
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Abstract
본 연구는 LC-ESI-MS/MS를 이용하여 혈청 중의 피나스테라이드의 분석법을 확립하고, 밸리데이션을 통하여 분석방법의 타당도를 검증하였다. 혈청에 내부표준물질로 베클로메타손을 첨가한 후 methyl tert-butyl ether (MTBE)을 사용하여 액체상추출법(liquid-liquid extraction, LLE)으로 전처리하였다. LC-MS/MS의 양이온 모드에서 피나스테라이드와 베클로메타손의 MRM (multiple reaction monitoring) mass transition은 각각 m/z 373.2→305.2, m/z 409.3→391.2 이었으며, 머무름 시간은 각각 5.81분, 5.46분이었다. 피나스테라이드의 정량한계는 0.1 ng/mL로 나타났으며, 0.1~20.0 ng/mL의 농도 범위에서 검량선은 우수한 직선성(R^2=0.9997)을 확인할 수 있었으며, 회수율은 80~83% 범위이었다. 피나스테라이드의 일내에 대한 정밀도는 6.3~10.6%, 정확도는 97.3~103.6% 범위이었으며, 연속 3일간 수행한 일간 정밀도는 0.8~5.2%,정확도는 99.8~102.5%로 나타났다.
- keywords
- Finasteride, LC-MS/MS, human serum
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