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- E-ISSN 2288-8985
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- E-ISSN2288-8985
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Determination of aromatic amino acids by chemiluminometric assay with Luminol-H2O2-Cu(II) system
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
2012, v.25 no.3, pp.171-177
https://doi.org/10.5806/AST.2012.25.3.171
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(2012). Determination of aromatic amino acids by chemiluminometric assay with Luminol-H2O2-Cu(II) system. Analytical Science and Technology, 25(3), 171-177, https://doi.org/10.5806/AST.2012.25.3.171
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Abstract
A determination method of aromatic amino acids such as trytophan (Trp), tyrosine (Tyr), and phenylalanine (Phe) using luminol-H2O2-Cu(II) system has been presented. In the presence of an aromatic amino acid, the enhanced chemiluminescence (CL) intensity of luminol-H2O2-Cu(II) system was obtained by forming a complex between Cu(II) and the amino acid. Based on the above phenomenon, a sensitive and fast determination of three aromatic amino acids was performed using the CL method in batch-type detection system. To optimize determination conditions, the kinetic influence of an aromatic amino acid on the luminol-H2O2-Cu(II) system and the effects of H2O2 and Cu(II) concentration, pH, and buffers were investigated. Under the optimized conditions, the calibration curve was linear over the range from 1.0 × 10−6 to 2.0 × 10−5 M for Trp,1.0 × 10−6 to 2.0 × 10−5 M for Try, and 2.0 × 10−6 to 2.0 × 10−5 M for Phe, respectively. In this range, reproducibility (RSD, n = 4) of Trp, Try, and Phe were 3.21%, 2.64%, and 2.48%, respectively. The limit of detection (3σ/s) was calculated to be 6.8 × 10−7 M for Trp, 5.7 × 10−7 M for Try, and 9.6 × 10−7 M for Phe.
- keywords
- luminol-<TEX>$H_2O_2$</TEX>-Cu(II), chemiluminescence, aromatic amino acid
Reference
1
1. T. McKee and J. R. McKee, ‘Biochemistry: An introduction’, 2nd Ed., McGraw-Hill, New York, 1999.
2
2. B. Alberts, D. Bray, K. Hopkin, A. Johnson, J. Lewis, M. Raff, K. Roberts and P. Walter, ‘Essential cell biology’, 2nd Ed., Garland Science, New York, 2003.
3
3. S. Broer and M. Palacin, Biochem. J., 436, 193-211 (2011).
4
4. W. Leuchtenberger, K. Huthmacher and K. Drauz, Appl. Microbiol. Biotechnol., 69, 1-8 (2005).
5
5. K. A. Massey, C. H. Blakeslee and H. S. Pitkow, Amino acids, 14, 271-300 (1998).
6
6. Z. Zhang, S. Zhang and X. Zhang, Anal. Chim. Acta., 541, 37-47 (2005).
7
7. A. M. Powe, S. Das, M. Lowry, B. El-Zahab, S. O. Fakayode, M. L. Geng, G. A. Baker, L. Wang, M. E. McCarroll, G. Patonay, M. Li, M. Aljarrah, S. Neal and I. M. Warner, Anal. Chem., 82, 4865-4894 (2010).
8
8. M. R. Sangi, D. Jayatissa, J. P. Kim and K. A. Hunter, Talanta, 62, 924-930 (2004).
9
9. J. Lv, Z. Zhang, J. Li and L. Luo, Forensic Sci. Int., 148, 15-19 (2005).
10
10. D. T. Bostick and D. M. Hercules, Anal. Chem., 47(3), 447-452 (1975).
11
11. I. Parejo, C. Codina, C. Petrakis and P. Kefalas, J. Pharmacol. Toxicol., 44, 507-512 (2000).
12
12. J. S. Lee and H. B. Lim, Bull. Korean Chem. Soc., 28(12), 2315-2318 (2007).
13
13. P. Fletcher, K. N. Andrew, A. C. Calokerinos, S. Forbes and P. J. Worsfold, Luminescence, 16, 1-23 (2001).
14
14. C. A. Marquette and L. J. Blum, Anal. Bioanal. Chem., 385, 546-554 (2006).
15
15. L. G. Gracia, A. M. G. Campana, J. F. H. Perez and F. J. Lara, Anal. Chim. Acta., 640, 7-28 (2009).
16
16. I. P. A. Morais, I. V. Toth and A. O. S. S. Rangel, Talanta, 66(2), 341-347 (2004).
17
17. K. Tsukagoshi, M. Sumiyama, R. Nakajima, M. Nakayama and M. Maeda, Anal. Sci., 14, 409-412 (1998).
18
18. Y. M. Liu and Z. L. Liu, Chinese Chem. Lett., 21, 856-859 (2010).
19
19. S. D. Solomon, M. Bahadory, A. V. Jeyarajasingam, S. A. Rutkowsky, C. Boritz and L. Mulfinger, J. Chem. Educ., 84(2), 322-325 (2007).
20
20. H. A. Mottola and D. P. Bendito, Anal. Chem., 66(12), 131-162 (1994).
21
21. S. Y. Liao and C. W. Whang, J. Chromatogr. A., 736, 247-254 (1996).
22
22. Z. Li, K. Li and S. Tong, Analyt. Lett., 32(5), 901-913 (1999).
23
23. S. Kanhathaisong, S. Rattanaphani, V. Pattanaphani and T. Manyum, Suranaree J. Sci. Technol., 18(2), 159-165 (2011).
24
24. O. Altun and S. Bilcen, Spectrochim. Acta. A., 75, 789-793 (2010).
25
25. D. Badocco, P. Pastore, G. Favaro and C. Macca, Talanta, 72, 249-255 (2007).
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