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Preparation of cross-linked silk fibroin film by γ-irradiation and their application as supports for human cell culture
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
2014, v.27 no.1, pp.27-33
https://doi.org/10.5806/AST.2014.27.1.27
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(2014). Preparation of cross-linked silk fibroin film by γ-irradiation and their application as supports for human cell culture. Analytical Science and Technology, 27(1), 27-33, https://doi.org/10.5806/AST.2014.27.1.27
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Abstract
This study described about preparation of the cross-linked silk fibroin (SF) film by γ-irradiation ofthe casted SF film, which is fabricated from aqueous solution regenerated via fibers of cocoons and theirapplication as supports for human cell culture. The properties of cross-linked SF film were evaluated by FTIRspectroscopy, contact angle, solubility to water, thermal analysis, surface area analyzer, and morphology viascanning electron microscopy (SEM). The cross-linked SF films were not dissolved in water and exhibited therough surface morphology, large surface area, and good thermal properties. The human fibroblast cell (CCD-986sk) and embryo kidney-ft cell were well growed on the surface of cross-linked SF film supports preparedby γ-irradiation. The cross-linked SF film prepared by γ-irradiation can be used as biomaterials for human cellculture.
- keywords
- cross-linked silk fibroin film, <TEX>${\gamma}$</TEX>-Irradiation, human cell culture, human fibroblast cell, human embryo kidney-ft cell
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