- P-ISSN 1225-0163
- E-ISSN 2288-8985
- Log In/Sign Up
- P-ISSN1225-0163
- E-ISSN2288-8985
- SCOPUS, ESCI, KCI
Article Detail
Home > Article Detail
Analysis of ethyl glucuronide (EtG) in Hair for the diagnosis of chronic alcohol abuse of Korean
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
2020, v.33 no.3, pp.151-158
https://doi.org/10.5806/AST.2020.33.3.151
&
(2020). Analysis of ethyl glucuronide (EtG) in Hair for the diagnosis of chronic alcohol abuse of Korean. Analytical Science and Technology, 33(3), 151-158, https://doi.org/10.5806/AST.2020.33.3.151
- Downloaded
- Viewed
Abstract
Alcohol, which can easily be obtained in the same way as ordinary beverages, is harmful enough to cause death due to excessive drinking and chronic alcohol intake, so it is important to maintain a proper amount of drinking and healthy drinking habits. In addition, the incidence of behavioral disturbances and impaired judgments that can be caused by chronic alcohol drinking of more than adequate amounts of alcohol is also significant. Accordingly it is very useful for forensic science to check whether the person involved is drunken or is alcoholism state in various accidents. Currently, in Korea, alcohol consumption is determined by detecting the level of alcohol or alcohol metabolism ‘ethyl glucuronide (EtG)’ in blood or urine samples. However, analysis of alcohol or EtG in blood or urine can only provide information about the current state of alcohol consumption because of a narrow window of detection time. Therefore, it is important to analyze the EtG as a long-term direct alcohol metabolite bio-marker in human hair and to investigate relationship between alcohol consumption and EtG concentration for the evaluation of chronic ethanol consumption. In this study, we established an analytical method for the detection of EtG in Korean hair efficiently and validated selectivity, linearity, limits of detection (LOD), limits of quantification (LOQ), matrix effect, recovery, process efficiency, accuracy and precision using liquid chromatography tandem mass spectrometry (LC-MS/MS). In addition, the assay performance was evaluated in Korean social drinker’s hair and the postmortem hair of a chronic alcoholism. The results of this study can be useful in monitoring the alcohol abuse of Korean in clinical cases and legal procedures related to custody and provide a useful tool to evaluate postmortem diagnosis of alcoholic ketoacidosis in forensics.
- keywords
- chronic alcohol abuse, alcohol metabolites, ethyl glucuronide (EtG), hair
Reference
1
1. P. Kintz, A. Salomone and M. Vincenti, ‘Hair Analysis in Clinical and Forensic Toxicology’, 1st Ed., Academic Press, 2015.
2
2. C. L. Crunelle, M. Yegles, A. L. van Nuijs, A. Covaci, M.D. Doncker, K. E. Maudens, B. Sabbe, G. Dom, W. E. Lambert, P. Michielsen and H. Neels, Drug Alcohol Depend., 134, 1-11 (2014).
3
3. A. Salomone, M.R. Baumgartner, T. Lombardo, E. Alladio, D. Di Corcia and M. Vincenti, Forensic Sci. Int., 267, 60-65 (2016).
4
4. Society of Toxicological and Forensic Chemistry, ‘Guide lines and Recommendations of the GTFCh : Appendix B-Requirements for the validation of analytical methods’, 76(3), 185-208 (2009).
5
5. Society of Toxicological and Forensic Chemistry, ‘Guidelines and Recommendations of the GTFCh :Appendix C-Requirements for the analysis of hair samples’, 76(3), 209-216 (2009).
6
6. R. Agius, T. Nadulski, H. G. Kahl and B. Dufaux, Forensic Sci. Int., 215, 32-37 (2012).
7
7. W. Schubert and R. Mattern, ‘Beurteilungskriterien:Urteilsbildung in der Medizinisch-Psychologischen Fahreignungsdiagnostik (German Driving Licence Regranting Guidelines)’, Kirschbaum Verlag Bonn, Germany, 178, 2009.
8
8. G. Tassoni, D. Mirtella, M. Zampi, L. Ferrante, M. Cippitelli, E. Cognigni, R. Froldi, and M. Cingolani, Forensic Sci. Int., 244, 16-19 (2014).
9
9. C. Stramesi, M. Polla, C. Vignali, A. Zucchella and A. Groppi, Forensic Sci. Int., 176, 34-37 (2008).
10
10. L. Politi, L. Morini, F. Leone and A. Polettini, Addiction, 101(10), 1408-1412 (2006).
11
11. I. Kerekes and M. Yegles, Ther. Drug Monit., 35, 527-529 (2013).
12
12. F. Pragst and M. A. Balikova, Clin. Chim. Acta., 370, 17-49 (2006).
13
13. L. Morini, A. Zucchella, A. Polettini, L. Politi and A. Groppi, Forensic Sci. Int., 198, 23-27 (2010).
14
14. B. M. R. Appenzeller, R. Agirman, P. Neuberg, M. Yegles and R. Wennig, Forensic Sci. Int., 173, 87-92 (2007).
15
15. R. Agius and P. Kintz, Drug Test. Anal., 2(8), 367-376(2010).
16
16. C. L. Crunelle, D. Cappelle, M. Yegles, M. D. Doncker, P. Michielsen, G. Dom, A. L. van Nuijs, K. E. Maudens, A. Covaci and H. Neels, Anal. Bioanal. Chem., 408, 2019-2025 (2016).
17
17. H. Kharbouche, M. Faouzi, N. Sanchez, J. B. Daeppen, M. Augsburger, P. Mangin, C. Staub and F. Sporkert, Int. J. Legal. Med., 126, 243-250 (2012).
18
18. R. Agius, T. Nadulski, H. G. Kahl and B. Dufaux, Forensic Sci. Int., 218, 10-14 (2012).
19
19. M. Hastedt, M. Buchner, M. Rothe, R. Gapert, S. Herre, F. Krumbiegel, M. Tsokos, T. Kienast, A. Heinz and S. Hartwig, Forensic Sci. Med. Pathol., 9, 471-477(2013).
20
20. V. Pirro, D. D. Corcia, F. Seganti, A. Salomone and M. Vincenti, Forensic Sci. Int., 232, 229-236 (2013).
21
21. F. T. Peter, O. H. Drummer and F. Musshoff, Forensic Sci. Int., 165, 216-224 (2007).
22
22. Society of Hair Testing, http://www/soht/org/consensus, Assessed May 2019.
Other articles from this issue
- Method comparison for analyzing formaldehyde in marker pen ink
- Screening and detection of methylisothiazolinone and chloromethylisothiazolinone in cosmetics by UPLC-MS/MS
- Study on simultaneous determination of aromatic material causing allergic in children’s products by GC-MSD
- A comparison study of crude protein contents obtained utilizing the Kjeldahl method and Dumas combustion method in foods
- Analysis of ethyl glucuronide (EtG) in Hair for the diagnosis of chronic alcohol abuse of Korean
- Comparison of vacuum metal deposition (VMD) and powder method for developing latent fingerprint on plastic envelope surface
- Erratum: Electrochemically polyaniline-coated microextraction needle for phthalates in water
- more
Recommanded Articles
This website recommended the use the Chrome browser for journal website.