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  • P-ISSN 1225-0163
  • E-ISSN 2288-8985

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    The impact of freeze-drying on the glycoproteomic profiles of human milk

    Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
    2020, v.33 no.4, pp.177-185
    https://doi.org/10.5806/AST.2020.33.4.177
    Won-Ho Hahn (Department of Pediatrics, School of Medicine, Soon Chun Hyang University, Seoul Hospital)
    Seong Phil Bae (Department of Pediatrics, School of Medicine, Soon Chun Hyang University, Seoul Hospital)
    Hookeun Lee (College of Pharmacy and Gachon Institute of Pharmaceutical Science, Gachon University)
    Jong-Moon Park (College of Pharmacy and Gachon Institute of Pharmaceutical Science, Gachon University)
    Suyeon Park (Department of Biostatistics, Soon Chun Hyang Medical Center)


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    Abstract

    Human milk (HM) glycoproteins play important roles protecting infants against various pathogens. Recently, freezing HM is reported to affect some glycoproteins and freeze-drying is suggested as an alternative method. However, the effects of freeze-drying on HM glycoproteins were not evaluated yet. Six fresh HM samples were collected from three healthy mothers at 15 and 60th days of lactation from each mother. Each sample was divided into frozen and freeze-dried subgroups yielding totally 12 samples, and the glycoproteomic analysis was performed by liquid chromatography mass spectrometry. The results were compared between samples of 15 and 60th days of lactation, and before and after the freeze-drying. Totally, 203 glycoproteins were detected. The glycoprotein levels were not different between two groups of 15/60th day of lactation and before/after freeze-drying groups (P > 0.050). In addition, significant correlation of glycoprotein levels was found between the different lactation stages (r = 0.897, P < 0.001) and the status of freeze-drying (r = 0.887, P < 0.001) in a partial correlation analysis. As no significant change of HM glycoproteins was not found after the freezedrying, we hope that introducing freeze-drying to HM banks is supported by the present study. This work was supported by the National Research Foundation (NRF) of Korea grant funded by the Korea government (MSIP) (No.2017R1D1A1B03034270; No.2020R1A2C1005082).

    keywords
    freeze-drying, human milk, lyophilization, mass spectrometry, glycoprotein, glycoproteomics


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