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  • 한국과학기술정보연구원(KISTI) 서울분원 대회의실(별관 3층)
  • 2024년 07월 03일(수) 13:30
 

  • P-ISSN1225-0163
  • E-ISSN2288-8985
  • SCOPUS, ESCI, KCI

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  • P-ISSN 1225-0163
  • E-ISSN 2288-8985

논문 상세

    Improved HPLC-UV method for determination of five synthetic dyes in Typha orientalis

    분석과학 / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
    2021, v.34 no.4, pp.160-171
    https://doi.org/10.5806/AST.2021.34.4.160
    Kyung Yuk Ko (National Institute of Food and Drug Safety Evaluation)
    Eun Young Choi (Herbal Medicine Research Division, National Institute of Food and Drug Safety Evaluation)
    Se Hee Jeong (National Institute of Food and Drug Safety Evaluation)
    Ock jin Paek (Herbal Medicine Research Division, National Institute of Food and Drug Safety Evaluation)
    Chan Lee (Department of Food Science and Technology, Chung-Ang University)
    Huijin Heo (Division of Food and Animal Science, Chungbuk National University)
    She-Wook Oh (Herbal Medicine Research Division, National Institute of Food and Drug Safety Evaluation)
    Chulhyun Lee (Herbal Medicine Research Division, National Institute of Food and Drug Safety Evaluation)
    Juhye Kang (Herbal Medicine Research Division, National Institute of Food and Drug Safety Evaluation)
    Sooyeul Cho (Herbal Medicine Research Division, National Institute of Food and Drug Safety Evaluation)
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    Abstract

    Synthetic azo dyes are used extensively in herbal medicines to render the medicines more visually attractive to consumers. This study developed and validated a rapid high-performance liquid chromatography (HPLC) method to determine whether synthetic colorants such as Tartrazine, Auramine O, Metanil yellow, Sunset yellow, and Orange II are used extensively in Typha orientalis. To increase the recovery of the synthetic dyes, this method employed containing 50 mM ammonium acetate in 70 % methanol at first extraction and 100 mM HCl in 70 % methanol at second extraction. Five synthetic pigments in Typha orientalis were separated by gradient elution with a mobile phase consisting of acetonitrile and 50 mM ammonium acetate in distilled water at ultra-violet (UV) detection 428 nm or 500 nm. Additionally, this study established the liquid chromatography tandem mass spectrometry (LC-MS/MS) method to confirm positive samples suspected by HPLC results. The HPLC-UV method had good linearity, indicating r2> 0.999. The recoveries of the samples spiked with three different concentration ranged from 73.8~91.5 %, and relative standard deviation values indicated 0.2~5.2 %. The established LC-MS/MS could successfully identify the synthetic pigments in herbal medicine samples. The study demonstrates that Typha orientalis adulterated by yellowish synthetic dyes can be successfully distinguished when using the HPLC-UV method.

    keywords
    herbal medicines, synthetic dyes, Typha orientalis, HPLC, LC-MS/MS


    참고문헌

    1

    1. B. Esen, T. Oymak and E. Dural, Int. J. Sci. Eng. Res., 9(8), 72-76 (2018).

    2

    2. M. Xu, B. Huang, F. Gao, C. Zhai, Y. Yang, L. Li, W. Wang and L. Shi, Front. Pharmcol., 10, Article 1446, 1-8 (2019).

    3

    3. K. Rovina, S. Siddiquee and S. M. Shaarani, Crit. Rev. Anal. Chem., 47(4), 309-324 (2017).

    4

    4. K. S. Rowe and K. J. Rowe, J. Pediatrics, 125(5), 691-698 (1994).

    5

    5. Y. S. Al-Degs, Food Chem., 117(3), 485-490 (2009).

    6

    6. T. Nagaraja and T. Desiraju, Food Chem. Toxicol., 31(1), 41-44 (1993).

    7

    7. I. S. Khan, M. N. Ali, R. Hamid and S. A. Ganie, Toxicol. Rep., 7, 370-375 (2020).

    8

    8. M. Solís, A. Solís, H. I. Pérez, N. Manjarrez and M. Flores, Process Biochem., 47(12), 1723-1748 (2012).

    9

    9. K. T. Chung, G. E. Fulk and M. Egan, Appl. Environ. Microbiol., 35(3), 558-562 (1978).

    10

    10. R. Anliker, Richardson M., Ed., The Royal Society of Chemistry. London, UK. 166-187 (1986).

    11

    11. J. C. Tung, W. C. Huang, J. C. Yang, G.Y. Chen, C. C. Fan, Y. C. Chien, P. S. Lin, S. C. C. Lung and W. C. Chang, Environ. Toxicol., 32(11), 2379-2391 (2017).

    12

    12. S. Parodi, L. Santi, P. Russo, A. Albini, D. Vecchio, M. Pala, L. Ottaggio and A. Carbone, J. Toxicol. Environ. Health, 9(5-6), 941-952 (1982).

    13

    13. T. N. T. Kim, T. T. Bui, A. T. Pham, V. T. Duong and T. H. G Le, J. Anal. Methods Chem., 2019, Article ID 8639528 (2019).

    14

    14. T. I. Tikhomirova, G. R. Ramazanova and V. V. Apyari, Food Chem., 221, 351-355 (2017).

    15

    15. G. Karanikolopoulos, A. Gerakis, K. Papadopoulou and I. Mastrantoni, Food Chem., 177, 197-203 (2015).

    16

    16. F. Martin, J. M. Oberson, M. Meschiari and C. Munari, Food Chem., 197, 1249-1255 (2016).

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