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Optimization of an extraction method for the simultaneous quantification of six active compounds in the aril part of Orostachys japonicus using HPLC–UV
Analytical Science and Technology / Analytical Science and Technology, (P)1225-0163; (E)2288-8985
2021, v.34 no.4, pp.153-159
https://doi.org/10.5806/AST.2021.34.4.153
(College of Pharmacy, Chungnam National University)
(College of Pharmacy, Chungnam National University)
(College of Pharmacy, Chungnam National University)
(Department of Pharmaceutical Engineering, Sangji University)
(College of Pharmacy, Chungnam National University)
(College of Pharmacy, Chungnam National University)
(College of Pharmacy, Chungnam National University)
(College of Pharmacy, Chungnam National University)
(Department of Pharmaceutical Engineering, Sangji University)
(College of Pharmacy, Chungnam National University)
(College of Pharmacy, Chungnam National University)
Dan,
G.
, Jin,
H.
K.
, Chong,
W.
C.
, Seo,
Y.
Y.
, Young,
H.
K.
, &
Jong-Seong,
K.
(2021). Optimization of an extraction method for the simultaneous quantification of six active compounds in the aril part of Orostachys japonicus using HPLC–UV. Analytical Science and Technology, 34(4), 153-159, https://doi.org/10.5806/AST.2021.34.4.153
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Abstract
In this study, we describe the development of a new high-performance liquid chromatography (HPLC) method for the simultaneous analysis of six bioactive compounds (including gallic acid, epicatechin 3-gallate, quercitrin, afzelin, quercetin, and kaempferol) from Orostachys japonicus. The extraction method was investigated and optimization of the extraction time (min), solvent composition (%), and solvent to material ratio were conducted. As a result, 30 min extraction with 50% methanol and 40:1 mL/g of solvent: material ratio achieved the highest extraction efficiency with a yield of 3.32 mg/g. Furthermore, the developed HPLC method was validated and the correlation coefficient (R) values were within the satisfactory range of 0.9995-0.9999 over the linearity range of 1.53-417 μg/mL. The limit of detection and limit of quantification for the six active components were between 0.03-0.08 μg/mL and 0.08-0.26 μg/mL, respectively. With these newly optimized and developed methods, four batches of O. japonicus were analyzed to confirm the high extraction efficiency of the method and the feasibility of an application.
- keywords
- HPLC, O. Japonicus, quality control, ultrasonic-assisted extraction, method validation
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